FUNCTIONAL EXPRESSION OF 2 NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTORS FROM CDNA CLONES IDENTIFIES A GENE FAMILY
FUNCTIONAL EXPRESSION OF 2 NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTORS FROM CDNA CLONES IDENTIFIES A GENE FAMILY
复制标题
DOI：
10.1073/pnas.84.21.7763
复制
发表时间：
1987-11-01
影响因子：
11.1
通讯作者：
PATRICK, J
中科院分区：
文献类型：
--
作者：
BOULTER, J;CONNOLLY, J;PATRICK, J
编码与α同源的蛋白质的基因家族。肌肉尼古丁乙酰胆碱受体亚基已确定在大鼠基因组。这些基因在已知含有功能性尼古丁受体的中枢和周围神经系统中转录。在本文中，我们证明了这些基因中的三个，我们称之为alpha3， alpha4和beta2，编码的蛋白质在非洲爪蟾卵母细胞中表达时形成功能性尼古丁乙酰胆碱受体。表达alpha3或alpha4蛋白与β 2蛋白结合的卵母细胞对乙酰胆碱产生强烈反应。仅表达alpha4蛋白的卵母细胞对乙酰胆碱反应较弱。这些受体被乙酰胆碱和尼古丁激活，并被毒3.1阻断。它们不会被。alpha阻挡。-班加罗毒素，它能阻断肌肉中的烟碱乙酰胆碱受体。因此，由α 3、α 4和β 2亚基组成的受体在药理学上与神经节型神经元烟碱乙酰胆碱受体相似。这些结果表明，α 3、α 4和β 2基因编码功能性烟碱乙酰胆碱受体亚基，这些亚基在大脑和周围神经系统中表达。
A family of genes coding for proteins homologous to the .alpha. subunit of the muscle nicotine acetylcholine receptor has been identified in the rat genome. These genes are transcribed in the central and peripheral nervous systems in areas known to contain functional nicotinic receptors. In this paper, we demonstrate that three of these genes, which we call alpha3, alpha4, and beta2, encode proteins that form functional nicotinic acetylcholine receptors when expressed in Xenopus oocytes. Oocytes expressing either alpha3 or alpha4 protein in combination with the beta2 protein produced a strong response to acetylcholine. Oocytes expressing only the alpha4 protein gave a weak response to acetylcholine. These receptors are activated by acetylcholine and nicotine and are blocked by Bungarus toxin 3.1. They are not blocked by .alpha.-bungarotoxin, which blocks the muscle nicotinic acetylcholine receptor. Thus, the receptors formed by the alpha3, alpha4, and beta2 subunits are pharmacologically similar to the ganglionic-type neuronal nicotinic acetylcholine receptor. These results indicate that the alpha3, alpha4, and beta2 genes encode functional nicotinic acetylcholine receptor subunits that are expressed in the brain and peripheral nervous system.