Non-invasive assessment of phosphate metabolism and oxidative capacity in working skeletal muscle in healthy young Chinese volunteers using (31)P Magnetic Resonance Spectroscopy.
Non-invasive assessment of phosphate metabolism and oxidative capacity in working skeletal muscle in healthy young Chinese volunteers using (31)P Magnetic Resonance Spectroscopy.
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使用 (31)P 磁共振波谱法对中国健康年轻志愿者工作骨骼肌的磷酸盐代谢和氧化能力进行无创评估。
作者：
Li M;Chen F;Wang H;Wu W;Zhang X;Tian C;Yu H;Liu R;Zhu B;Zhang B;Dai Z
背景一般来说，男性在执行任务时比女性表现出更大的力量和肌肉能力。肌肉活检被认为是评价肌肉功能的参考方法，但它具有侵入性，存在采样误差，并且不适用于纵向研究和切除过程中的动态测量。在这项研究中，我们建立了一个内部力控制和测量系统，用于在受试者接受31 P磁共振谱（31 P-MRS）时对股四头肌定量施加力;我们的目的是调查年轻健康中国志愿者工作肌肉中磷酸盐代谢物变化是否存在性别差异。方法.志愿者在飞利浦3 T磁共振（MR）扫描仪中俯卧时使用力控制和测量系统进行膝盖伸展运动。将31 P-MRS线圈牢固放置在股四头肌中部下方。分别在运动前、运动中、运动后采集股四头肌31 P-MRS测量无机磷（Pi）、磷酸肌酸（PCr）和三磷酸腺苷（ATP）。比较了不同性别的PCr、Pi、PCr/Pi、PCr/ATP、pH、功能比、kPCr和氧化能力。结果共有17名志愿者参加了这项研究。男性10例，年龄23.30 ± 1.25岁;女性7例，年龄23.57 ± 0.79岁。在本研究中，男性的WE显著高于女性（16.33 ± 6.46 vs. 7.82 ± 2.16，p = 0.002）。在不同运动状态下PCr、Pi、PCr/Pi、PCr/ATP、pH、kPCr和氧化能力中，只有PCr/Pi、PCr/ATP、pH、kPCr和氧化能力在不同运动状态下，（运动时，男性= 5.630 ± 1.647，女性= 4.014 ± 1.298，p = 0.047），PCr/ATP（运动期间，男性=1.273 ± 0.219，女性= 1.523 ± 0.167，p = 0.025），ATP（运动后，男性= 24.469 ± 3.911 mmol/kg，女性= 18.353 ± 4.818 mmol/kg，p = 0.035）有显著的性别差异。运动过程中，男性的PCr/Pi显著高于女性，但PCr/ATP低于女性，表明男性的能量传递效率高于女性。运动后PCr恢复无性别差异。结论.我们的内部力控制和测量系统定量施加的力量在运动过程中的31 P-MRS实验，和较高的能量传递效率和WE的性别差异检测男性轻度负荷运动股四头肌。这种无创性的技术使我们能够在未来进一步研究和了解高能磷酸盐代谢的性别差异。
Background. Generally, males display greater strength and muscle capacity than females while performing a task. Muscle biopsy is regarded as the reference method of evaluating muscle functions; however, it is invasive and has sampling errors, and is not practical for longitudinal studies and dynamic measurement during excise. In this study, we built an in-house force control and gauge system for quantitatively applying force to quadriceps while the subjects underwent 31P Magnetic Resonance Spectroscopy (31P-MRS); our aim was to investigate if there is a sex difference of phosphate metabolite change in working muscles in young heathy Chinese volunteers. Methods. Volunteers performed knee-extending excises using a force control and gauge system while lying prone in a Philips 3T Magnetic Resonance (MR) scanner. The 31P-MRS coil was firmly placed under the middle of the quadriceps . 31P-MRS measurements of inorganic phosphate (Pi), phosphocreatine (PCr) and adenosine triphosphate (ATP) were acquired from quadriceps while subjects were in a state of pre-, during- and post-exercise. The PCr, Pi, PCr/Pi, PCr/ATP, pH, work/energy cost ratio (WE), kPCr and oxidative capacity were compared between males and females. Results. A total of 17 volunteers underwent the study. Males: N = 10, age = 23.30 ± 1.25years; females: N = 7, age = 23.57 ± 0.79 years. In this study, males had significantly greater WE (16.33 ± 6.46 vs. 7.82 ± 2.16, p = 0.002) than females. Among PCr, Pi, PCr/Pi, PCr/ATP, pH, kPCr and oxidative capacity at different exercise status, only PCr/Pi (during-exercise, males = 5.630 ± 1.647, females = 4.014 ± 1.298, p = 0.047), PCr/ATP (during-exercise, males =1.273 ± 0.219, females = 1.523 ± 0.167, p = 0.025), and ATP (post-exercise, males = 24.469 ± 3.911 mmol/kg, females = 18.353 ± 4.818 mmol/kg, p = 0.035) had significant sex differences. Males had significantly greater PCr/Pi, but less PCr/ATP than females during exercise, suggesting males had higher energy transfer efficiency than females. At the post-exercise status, the recovery of PCr did not show sex difference. Conclusions. Our in-house force control and gauge system quantitatively applied force during the exercise for 31P-MRS experiments, and a sex difference of higher energy transfer efficiency and WE was detected in males with mild loaded exercising quadriceps. This noninvasive technology allows us to further study and understand the sex difference of high energy phosphate metabolism in the future.