SIMILARITY OF EFFECTS OF VASOPRESSIN, ADENOSINE-3',5'-PHOSPHATE (CYCLIC AMP) AND THEOPHYLLINE ON TOAD BLADDER
SIMILARITY OF EFFECTS OF VASOPRESSIN, ADENOSINE-3',5'-PHOSPHATE (CYCLIC AMP) AND THEOPHYLLINE ON TOAD BLADDER
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DOI：
10.1172/jci104528
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发表时间：
1962-01-01
影响因子：
15.9
通讯作者：
HANDLER, JS
中科院分区：
文献类型：
--
作者：
ORLOFF, J;HANDLER, JS
研究了腺苷-3、磷酸腺苷- 5（环磷酸腺苷）、茶碱和加压素对蟾蜍膀胱水通透性和短路电流（钠转运）的影响。在分离的蟾蜍膀胱囊的浆膜表面添加这些物质均匀地增加了水穿过膜的渗透流量。环AMP和茶碱所增加的通透性在各方面都与抗利尿激素相似。渗透性的变化是可逆的，不是通过在膀胱粘膜表面添加试验剂而产生的，并且与短路电流和跨膜电位差的升高有关。n -乙基马来酰亚胺，一种巯基抑制剂，可阻止环AMP、茶碱和抗利尿激素的作用。另一方面，洗浴液的酸化会干扰抗利尿激素和茶碱的作用，但不会干扰环AMP的作用。鉴于环AMP对其他激素作用的已知作用，环AMP、茶碱和抗利尿激素在蟾蜍膀胱中的作用具有明显的相似性，这与抗利尿激素在蟾蜍膀胱中起作用，在肾脏中起作用的观点是一致的。通过刺激受体组织中环状AMP的产生和积累。目前尚不清楚环AMP引发了哪些物理和生化事件，这些事件最终导致膜结构的改变，从而使水沿着渗透梯度加速流动。
The effect of adenosine-3[image], 5[image]-phosphate (cyclic AMP), theophylline, and vasopressin on the permeability to water and the short circuit current (sodium transport) of toad bladder was investigated. The addition of each of these substances to the serosal surface of the isolated toad bladder sac uniformly increases the osmotic flow of water across the membrane. The increase in permeability provided by cyclic AMP and theophylline resembles in all respects that due to vasopressin. The change in permeability is reversible, is not produced by the addition of the test agent to the mucosal surface of the bladder and is associated with a rise in both short circuit current and potential difference across the membrane. N-ethylmaleimide, a sulfhydryl inhibitor, prevents the effect of cyclic AMP, theophylline, and vasopressin. Acidification of the bathing solutions, on the other hand, interferes with the action of vasopressin and theophylline, but not with that of cyclic AMP. In view of the known role of cyclic AMP in the action of other hormones, the marked similarity of effects of cyclic AMP, theophylline, and vasopressin in toad bladder is consistent with the view that vasopressin exerts its effect in toad bladder, and by analogy in kidney, by stimulating the production and accumulation of cyclic AMP in the receptor tissue. It is not known what physical and biochemical events are initiated by cyclic AMP which ultimately result in an alteration in membrane structure that permits the accelerated flow of water along an osmotic gradient.