The CBF1-dependent low temperature signalling pathway, regulon and increase in freeze tolerance are conserved in Populus spp.
The CBF1-dependent low temperature signalling pathway, regulon and increase in freeze tolerance are conserved in Populus spp.
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DOI：
10.1111/j.1365-3040.2006.01505.x
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发表时间：
2006-07-01
影响因子：
7.3
通讯作者：
Hurry, Vaughan
中科院分区：
文献类型：
--
作者：
Benedict, Catherine;Skinner, Jeffrey S.;Hurry, Vaughan
温带木本多年生植物的分生组织必须适应寒冷的温度才能越冬并在第二年恢复生长。为了确定是否C-重复序列结合因子（CBF）家族的转录因子在一年生草本植物的这一过程中也在木本多年生植物的功能，我们研究了表型和转录谱的变化，从拟南芥（AtCBF 1）的转基因杨树组成型表达CBF 1。AtCBF 1的异位表达足以显着增加非驯化的叶和茎相对于野生型植物的抗冻性。cDNA微阵列实验确定了在杨树异位AtCBF 1表达上调的基因，表现出很强的保守性的CBF调节子之间的杨树和拟南芥，并确定叶和茎调节子之间的差异。我们研究了4个从杨树中鉴定的CBF旁系同源物的诱导动力学和组织特异性。子基因组序列（PtCBFs）。所有四种PtCBF在叶片中都是冷诱导的，但只有PtCBF 1和PtCBF 3在茎中表现出显著的诱导。我们的研究结果表明，CBF家族的转录激活因子在拟南芥的冷驯化中发挥的核心作用已在杨树。然而，PtCBFs的差异表达和CBF响应基因的不同集群在一年生（叶）和多年生（茎）组织表明，常年驱动的冬季休眠的演变可能已经引起了特定的角色，这些“主开关”在不同的一年生和多年生木本植物组织。
The meristematic tissues of temperate woody perennials must acclimate to freezing temperatures to survive the winter and resume growth the following year. To determine whether the C-repeat binding factor (CBF) family of transcription factors contributing to this process in annual herbaceous species also functions in woody perennials, we investigated the changes in phenotype and transcript profile of transgenic Populus constitutively expressing CBF1 from Arabidopsis (AtCBF1). Ectopic expression of AtCBF1 was sufficient to significantly increase the freezing tolerance of non-acclimated leaves and stems relative to wild-type plants. cDNA microarray experiments identified genes up-regulated by ectopic AtCBF1 expression in Populus, demonstrated a strong conservation of the CBF regulon between Populus and Arabidopsis and identified differences between leaf and stem regulons. We studied the induction kinetics and tissue specificity of four CBF paralogues identified from the Populus balsamifera subsp. trichocarpa genome sequence (PtCBFs). All four PtCBFs are cold-inducible in leaves, but only PtCBF1 and PtCBF3 show significant induction in stems. Our results suggest that the central role played by the CBF family of transcriptional activators in cold acclimation of Arabidopsis has been maintained in Populus. However, the differential expression of the PtCBFs and differing clusters of CBF-responsive genes in annual (leaf) and perennial (stem) tissues suggest that the perennial-driven evolution of winter dormancy may have given rise to specific roles for these 'master-switches' in the different annual and perennial tissues of woody species.