Determining Maximum Glycolytic Capacity Using Extracellular Flux Measurements.
Determining Maximum Glycolytic Capacity Using Extracellular Flux Measurements.
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DOI：
10.1371/journal.pone.0152016
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Brand MD
中科院分区：
文献类型：
--
作者：
Mookerjee SA;Nicholls DG;Brand MD
使用细胞外通量分析测量糖酵解速率和最大糖酵解能力可以提供关于正常操作、病理发展、分化和恶性转化期间细胞状态和表型的关键信息。它们在评估化学或药物治疗的效果时也很有用。在这里，我们实验定义最大糖酵解能力，证明它是如何不同于糖酵解速率，并提供了一个协议，用于确定基础糖酵解速率和最大糖酵解能力的细胞外通量测量。这些结果说明了细胞外通量分析的力量，以完全定量的方式描述培养中贴壁细胞的能量学。
Measurements of glycolytic rate and maximum glycolytic capacity using extracellular flux analysis can give crucial information about cell status and phenotype during normal operation, development of pathology, differentiation, and malignant transformation. They are also of great use when assessing the effects of chemical or drug treatments. Here, we experimentally define maximum glycolytic capacity, demonstrate how it differs from glycolytic rate, and provide a protocol for determining the basal glycolytic rate and maximum glycolytic capacity in cells using extracellular flux measurements. The results illustrate the power of extracellular flux analysis to describe the energetics of adherent cells in culture in a fully quantitative way.