PREPARATION OF SUSPENSIONS OF WASHED PLATELETS FROM HUMANS
PREPARATION OF SUSPENSIONS OF WASHED PLATELETS FROM HUMANS
复制标题
DOI：
10.1111/j.1365-2141.1972.tb08800.x
复制
发表时间：
1972-01-01
影响因子：
6.5
通讯作者：
ARDLIE, NG
中科院分区：
文献类型：
--
作者：
MUSTARD, JF;PACKHAM, MA;ARDLIE, NG
已经开发了用于制备来自人的洗涤血小板的悬浮液的方法。在清洗液中使用肝素以防止：凝血酶生成，使用腺苷三磷酸双磷酸酶以防止腺嘌呤核苷酸蓄积。悬浮在含白蛋白的Eagle组织培养基中的血小板比Tyrode-白蛋白溶液中的血小板对ADP的反应性更强。需要添加纤维蛋白原以获得对ADP诱导的聚集的最大敏感性。这些血小板可以在37°C下在腺苷三磷酸双磷酸酶存在下储存4小时或更长时间，并且在加入低浓度ADP时保持其聚集的能力。没有腺苷三磷酸双磷酸酶，血小板在37°C下储存时逐渐变得对ADP不敏感;这可能是由于ADP在悬浮液中的积累引起的，因为通过加入腺苷三磷酸双磷酸酶和进一步孵育可以部分恢复敏感性。
Methods have been developed for the preparation of suspensions of washed platelets from humans. Heparin is used in the washing fluids to prevent: thrombin generation and apyrase is used to prevent adenine nucleotide accumulation. Platelets suspended in Eagle's tissue culture medium containing albumin were more responsive to ADP than platelets in Tyrode's‐albumin solution. Addition of fibrinogen is required for maximum sensitivity to ADP‐induced aggregation. These platelets can be stored for 4 hr or more at 37°C in the presence of apyrase and maintain their ability to aggregate upon the addition of low concentrations of ADP.Without apyrase the platelets gradually become insensitive to ADP upon storage at 37°C; this is presumably caused by the accumulation of ADP in the suspending fluid because sensitivity can be partially restored by the addition of apyrase and further incubation.