MORPHOLOGICAL AND CYTOSKELETAL CHANGES IN EPITHELIAL-CELLS OCCUR IMMEDIATELY UPON INTERACTION WITH SALMONELLA-TYPHIMURIUM GROWN UNDER LOW-OXYGEN CONDITIONS
MORPHOLOGICAL AND CYTOSKELETAL CHANGES IN EPITHELIAL-CELLS OCCUR IMMEDIATELY UPON INTERACTION WITH SALMONELLA-TYPHIMURIUM GROWN UNDER LOW-OXYGEN CONDITIONS
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DOI：
10.1111/j.1365-2958.1992.tb01765.x
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发表时间：
1992-11-01
影响因子：
3.6
通讯作者：
FALKOW, S
中科院分区：
文献类型：
--
作者：
FRANCIS, CL;STARNBACH, MN;FALKOW, S
发现在限氧条件下生长的鼠伤寒沙门氏菌在感染后15分钟内进入HEp-2细胞，引起肌动蛋白丝重排，并影响HEp-2细胞的形态变化。视频显微镜显示，宿主细胞的形态变化与进入1分钟内开始生产性坚持。极化的Caco-2细胞形态受到低氧生长的S.鼠伤寒。静止相S.鼠伤寒沙门氏菌在这段时间内没有引起这些现象，即使用无菌毛粘附素AFA-I增强粘附。因此，环境线索调节S。鼠伤寒侵袭因子，允许立即进入宿主细胞。此外，真核宿主细胞中的肌动蛋白丝重排和形态学变化对于进入是必不可少的，并且在感染后几分钟内发生。
Salmonella typhimurium grown under oxygen-limiting conditions were found to enter into, elicit actin filament rearrangement in, and effect morphological changes upon HEp-2 cells within 15 min after infection. Video microscopy revealed that host cell morphological changes associated with entry began within 1 min of productive adherence. Polarized Caco-2 cell morphology was affected 40 s after infection with low-oxygen-grown S. typhimurium. Stationary-phase S. typhimurium did not elicit these phenomena within this time-period even when adherence was enhanced with the afimbrial adhesin, AFA-I. Thus, environmental cues regulate S. typhimurium invasion factors, allowing for immediate entry into host cells. Additionally, actin filament rearrangement and morphological changes in the eukaryotic host cell are essential for entry and occur within minutes of infection.