Regeneration of uterine horns in rats by collagen scaffolds loaded with collagen-binding human basic fibroblast growth factor
Regeneration of uterine horns in rats by collagen scaffolds loaded with collagen-binding human basic fibroblast growth factor
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DOI：
10.1016/j.biomaterials.2011.07.050
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发表时间：
2011-11-01
期刊：
影响因子：
14
通讯作者：
Hu, Yali
中科院分区：
文献类型：
--
作者：
Li, Xinan;Sun, Haixiang;Hu, Yali
子宫内膜严重损伤，阻碍胚胎着床和胎盘形成，常导致不孕或妊娠并发症。由于子宫本身材料有限，子宫功能及内环境复杂，目前尚无有效的治疗方法。在本研究中，胶原靶向碱性成纤维细胞生长因子（bFGF）的传递系统构建的胶原蛋白膜负载bFGF融合胶原蛋白结合域（CBD）的N-末端，限制bFGF从胶原蛋白的扩散。我们在大鼠严重子宫损伤模型（部分大鼠子宫角切除/重建）下测试了bFGF递送系统，发现该递送系统改善了大鼠子宫内膜和肌细胞的再生能力，改善了血管化，以及更好的妊娠结局。因此，这种靶向给药系统可能是一种有效的子宫组织再生策略。(C)2011爱思唯尔有限公司保留所有权利。
Severe damages of uterine endometrium which prevent embryos from implantation and placentation finally often result in infertility or pregnant complications. There is lack of effective treatments due to the limitation of native materials available and complexity of the function and internal environment of uterus. In the present study, a collagen targeting basic fibroblast growth factor (bFGF) delivery system was constructed by a collagen membrane loaded with bFGF fused a collagen-binding domain (CBD) to the N-terminal which limits the diffusion of bFGF from collagen. We tested the bFGF delivery system in rats under the severe uterine damage model (partial rat uterine horn excision/reconstruction), and found this delivery system improved regeneration abilities of uterine endometrium and muscular cells, improved vascularization, as well as better pregnancy outcomes in rats. Therefore, this targeting delivery system may be an effective strategy for uterine tissue regeneration. (C) 2011 Elsevier Ltd. All rights reserved.