Interaction of Nup53 with Ndc1 and Nup155 is required for nuclear pore complex assembly
Interaction of Nup53 with Ndc1 and Nup155 is required for nuclear pore complex assembly
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DOI：
10.1242/jcs.141739
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发表时间：
2014-02-15
影响因子：
4
通讯作者：
Antonin, Wolfram
中科院分区：
文献类型：
--
作者：
Eisenhardt, Nathalie;Redolfi, Josef;Antonin, Wolfram
核孔复合物（NPCs）是核胞质交换的通道。这些巨大的复合体由几百个单独的组分有序地组装成一个复杂的蛋白质相互作用网络，使核膜的两层膜变形成一个孔，人们对这一点只是初步的了解。在这里，我们发现Nup53和完整孔膜蛋白Ndc1之间的相互作用对于脊椎动物NPC组装至关重要。Nup53上的Ndc1结合位点与诱导膜弯曲的区域重叠，并且特别需要调节这种活性，这表明Nup53的膜变形能力在NPC组装过程中被调节。我们进一步证明，Nup53和Nup155的相互作用在NPC形成中起着至关重要的作用，是Nup155招募到组装孔的主要决定因素。总的来说，我们的研究结果明确了Nup53完成的相互作用模式的多样性，强调了该蛋白是孔膜和NPC之间的重要联系，也是孔膜形成的关键因素。
Nuclear pore complexes (NPCs) are the gateways for nucleocytoplasmic exchange. The ordered assembly of these huge complexes from several hundred individual components into an intricate protein interaction network which deforms the two membranes of the nuclear envelope into a pore is only rudimentarily understood. Here, we show that the interaction between Nup53 and the integral pore membrane protein Ndc1 is essential for vertebrate NPC assembly. The Ndc1 binding site on Nup53 overlaps with a region that induces membrane bending and is specifically required to modulate this activity, suggesting that the membrane-deforming capability of Nup53 is adjusted during the NPC assembly process. We further demonstrate that the interaction of Nup53 and Nup155 has a crucial role in NPC formation as the main determinant of recruitment of Nup155 to the assembling pore. Overall, our results pinpoint the diversity of interaction modes accomplished by Nup53, highlighting this protein as an essential link between the pore membrane and the NPC, and as a crucial factor in the formation of the pore membrane.