RAPID IDENTIFICATION OF PERIODONTAL PATHOGENS IN SUBGINGIVAL PLAQUE - COMPARISON OF INDIRECT IMMUNOFLUORESCENCE MICROSCOPY WITH BACTERIAL CULTURE FOR DETECTION OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS
RAPID IDENTIFICATION OF PERIODONTAL PATHOGENS IN SUBGINGIVAL PLAQUE - COMPARISON OF INDIRECT IMMUNOFLUORESCENCE MICROSCOPY WITH BACTERIAL CULTURE FOR DETECTION OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS
复制标题
DOI：
10.1177/00220345850640050201
复制
发表时间：
1985-01-01
影响因子：
7.6
通讯作者：
NEIDERS, ME
中科院分区：
文献类型：
--
作者：
BONTA, Y;ZAMBON, JJ;NEIDERS, ME
与选择性或非选择性培养相比，用特异性多克隆或单克隆血清诊断试剂间接免疫荧光显微镜检测龈下菌斑中伴生放线杆菌的敏感性为82-100%。在局限性青少年牙周炎患者中，100%的牙周病变部位发现了这种细菌，并与牙周疾病的临床指标(牙龈指数、菌斑指数和牙袋深度)有统计学上的相关性。间接免疫荧光显微镜技术是一种快速、可靠的检测牙菌斑中伴生放线菌的技术，可用于临床诊断、治疗和监测伴生放线菌牙周炎。
The sensitivity of indirect immunofluorescence microscopy using specific polyclonal or monoclonal serodiagnostic reagents for Actinobacillus actinomycetemcomitans in subgingival dental plaque ranged from 82-100% as compared with culture on selective or non-selective media. This bacterium was found in 100% of the periodontally diseased sites examined in localized juvenile periodontitis patients and was statistically related to clinical indices of periodontal disease including the gingival index, plaque index and pocket depth. Indirect immunofluorescence microscopy is a useful technique for the rapid and reliable determination of A. actinomycetemcomitans in human subgingival dental plaque which may be applied to clinical diagnosis, treatment and monitoring of periodontitis associated with A. actinomycetemcomitans.