Variations of tandem repeat, regulatory element, and promoter regions revealed by wheat-rye amphiploids
Variations of tandem repeat, regulatory element, and promoter regions revealed by wheat-rye amphiploids
复制标题
小麦-黑麦双倍体揭示的串联重复序列、调控元件和启动子区域的变异。
DOI：
10.1139/g08-027
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发表时间：
2008-06-01
期刊：
影响因子：
3.1
通讯作者：
Zhang, Huai-Qiong
中科院分区：
文献类型：
--
作者：
Tang, Zong-Xiang;Fu, Shu-Lan;Zhang, Huai-Qiong
为了更好地了解异源多倍体的进化，用4个不同的组合对小麦(Triticum aestivum L.)和黑麦(Secale graale L.)利用150个小麦SSR(Single Sequence Repeat，单序列重复)标记和以黑麦特异重复序列(PSc200)为探针的FISH分析，对12个F1和12个衍生的双倍体进行了分析，并与其直接亲本进行了比较。9个SSR标记扩增出471~1089个碱基的黑麦特异片段。这些片段含有调控元件和(或)启动子。其中一些片段是从所有24个子代中扩增出来的，而另一些片段是从子代中扩增出来的。一些后代的黑麦特异片段的消失是由序列消除或DNA修饰引起的。标记Xgwm320从一些后代中扩增出一个新片段(403bp)，这是一个黑麦特有的串联重复序列。有28个SSR标记在‘中国春’ב锦州黑麦’后代中表现出微卫星变异，而在其他3个组合的后代中，150个SSR标记均未表现出微卫星变异。在异源多倍化过程中，pSc200的FISH信号从‘Kustro’4条染色体的一个端粒/亚端粒中消失，并在‘中国春’בAR106BONE’衍生的双倍体中扩增。因此，小麦-黑麦的异源多倍化可能伴随着串联重复序列、调控元件和启动子区域的快速变异。重复序列pSc200的改变表明新形成的双倍体的组成基因组之间存在协调。在异源多倍化过程中，亲本不同的遗传背景似乎会影响基因组的变化。
To better understand the evolution of allopolyploids, 4 different combinations between wheat (Triticum aestivum L.) and rye (Secale cereale L.) including 12 F1 hybrids and 12 derived amphiploids were analyzed and compared with their direct parental plants by PCR analysis using 150 wheat SSR (single sequence repeat) markers and by FISH analysis using a rye-specific repetitive sequence (pSc200) as a probe. Nine SSR markers amplified rye-specific fragments whose sizes ranged from 471 bp to 1089 bp. These fragments contain regulatory elements and (or) promoters. Some of these fragments were amplified from all 24 progenies, while others were amplified from a subset of the progenies. The disappearance of rye-specific fragments from some progenies was caused by sequence elimination or DNA modification. Marker Xgwm320 amplified a new fragment (403 bp), a rye-specific tandem repeat, from some of the progenies. Twenty-eight SSR markers displayed microsatellite variation in progenies derived from 'Chinese Spring' x 'Jinzhou-heimai', but none of the 150 SSR markers displayed microsatellite variation in the progenies derived from the other three combinations. FISH signals of pSc200 were eliminated from one telomere/subtelomere of 4 chromosomes of 'Kustro' during allopolyploidization and expanded in amphiploids derived from 'Chinese Spring' x 'AR106BONE'. Thus, allopolyploidization in wheat-rye can be accompanied by rapid variation of tandem repeats, regulatory elements, and promoter regions. The alterations of repetitive sequence pSc200 indicate coordination between the constituent genomes of the newly formed amphiploids. Different genetic backgrounds of parents appear to affect genome changes during allopolyploidization.