Mirror-symmetric microtubule assembly and cell interactions drive lumen formation in the zebrafish neural rod
Mirror-symmetric microtubule assembly and cell interactions drive lumen formation in the zebrafish neural rod
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DOI：
10.1038/emboj.2012.305
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发表时间：
2013-01-09
期刊：
影响因子：
11.4
通讯作者：
Clarke, Jonathan D. W.
中科院分区：
文献类型：
--
作者：
Buckley, Clare E.;Ren, Xiaoyun;Clarke, Jonathan D. W.
通过分析细胞和亚细胞事件发生在中心的发展中的斑马鱼神经杆，我们已经发现了一种新的机制，细胞极化管腔形成过程中。来自神经杆两侧的细胞交叉穿过组织中线。这对于将顶端连接蛋白定位到细胞与组织中线相交的区域是必要的。细胞围绕组织中线组装镜像对称的微管细胞骨架，这对于正常管腔形成所需的蛋白质的运输是必要的，例如将缺陷3和Rablla分配到这一点。这提前发生，并且独立于中线细胞分裂，中线细胞分裂已被证明在管腔组织中具有强大的作用。据我们所知，这是第一个顶端极化开始的例子部分沿着细胞的长度，而不是在细胞的极端。虽然中线分裂不是顶端极化所必需的，但它通过有效地消除细胞过程而赋予形态发生优势，否则这些细胞过程将桥接发育中的管腔。The EMBO Journal（2013）32，30-44. doi：10.1038/daj.2012.305; 2012年11月30日在线发布
By analysing the cellular and subcellular events that occur in the centre of the developing zebrafish neural rod, we have uncovered a novel mechanism of cell polarisation during lumen formation. Cells from each side of the neural rod interdigitate across the tissue midline. This is necessary for localisation of apical junctional proteins to the region where cells intersect the tissue midline. Cells assemble a mirror-symmetric microtubule cytoskeleton around the tissue midline, which is necessary for the trafficking of proteins required for normal lumen forma:ton, such as partitioning defective 3 and Rablla to this point. This occurs in advance and is independent of the midline cell division that has been shown to have a powerful role in lumen organisation. To our knowledge, this is the first example of the initiation of apical polarisation part way along the length of a cell, rather than at a cell extremity. Although the midline division is not necessary for apical polarisation, it confers a morphogenetic advantage by efficiently eliminating cellular processes that would otherwise bridge the developing lumen. The EMBO Journal (2013) 32, 30-44. doi:10.1038/emboj.2012.305; Published online 30 November 2012