FASCICLIN-I AND FASCICLIN-II HAVE DISTINCT ROLES IN THE DEVELOPMENT OF GRASSHOPPER PIONEER NEURONS
FASCICLIN-I AND FASCICLIN-II HAVE DISTINCT ROLES IN THE DEVELOPMENT OF GRASSHOPPER PIONEER NEURONS
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DOI：
10.1016/0896-6273(93)90146-i
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发表时间：
1993-09-01
期刊：
影响因子：
16.2
通讯作者：
JAY, DG
中科院分区：
文献类型：
--
作者：
DIAMOND, P;MALLAVARAPU, A;JAY, DG
我们已经使用了一种新的技术，微CALI（发色团辅助激光灭活），研究神经细胞粘附分子fasciclin I和II在蚱蜢Ti1神经元的发展中的功能。Fasciclin I的微CALI导致T11轴突的去纤维化，类似于使用大规模卡利实现的结果（Jay和Keshishian，1990）。轴突分离的起始点对应于激光照射的部位，并且去束总是在该点的远端继续。Fasciclin II的微CALI阻止了Ti 1轴突生长的起始，但对成束没有影响。这种效应仅限于胞质分裂和生长锥出现之间的3小时间隔。
We have used a new technique, micro-CALI (chromophore-assisted laser inactivation), to investigate the function of the neural cell adhesion molecules fasciclin I and II in the development of the grasshopper Ti1 neurons. Micro-CALI of fasciclin I results in defasciculation of the Ti1 axons similar to that achieved using large scale CALI (Jay and Keshishian, 1990). The initial point of axon separation corresponds to the site of laser irradiation, and defasciculation always continues distal to this point. Micro-CALI of fasciclin II prevents the initiation of Ti1 axon outgrowth but has no effect on fasciculation. This effect is restricted to a 3 hr interval between cytokinesis and growth cone emergence.