Retardation of photoreceptor degeneration in the detached retina of rd1 mouse.
Retardation of photoreceptor degeneration in the detached retina of rd1 mouse.
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DOI：
10.1167/iovs.07-0715
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发表时间：
2008-02
影响因子：
4.4
通讯作者：
Hiroki Kaneko;K. Nishiguchi;Makoto Nakamura;S. Kachi;H. Terasaki
中科院分区：
文献类型：
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作者：
Hiroki Kaneko;K. Nishiguchi;Makoto Nakamura;S. Kachi;H. Terasaki
目的研究实验性视网膜脱离（RD）对视网膜光感受器变性的保护作用。方法在出生后第9天（P），在rd 1小鼠眼内制作RD。收集这些眼睛并与无RD的对照进行比较。通过对视网膜切片和平板标本中P30时外核层（ONL）、视杆细胞和视锥细胞以及视网膜血管的细胞核进行组织化学染色，评价RD对视网膜变性的影响。在P15通过TdT介导的dUTP缺口末端标记（TUNEL）检测凋亡的光感受器。还将有或无RD的小鼠在黑暗中饲养，并在P30时进行化学评价。结果在P30时，与无RD的眼相比，RD 1眼ONL中的视紫红质阳性（视杆）、花生凝集素阳性（视锥）和二氨基-2-苯基吲哚染色（视杆+视锥）细胞的数量分别增加了2.0倍、1.3倍和1.2倍。在视网膜脱离中，视锥细胞内/外节结构和围绕内核层和ONL的视网膜深血管被保留，但不包括节细胞层。在P15，ONL中的TUNEL阳性细胞数量在RD眼中显著减少。光暴露对有或没有RD的眼睛的感光细胞变性没有影响。结论：RD通过降低rd 1小鼠光感受器的凋亡率介导ONL和周围血管结构中的视锥和视杆细胞的保护。光剥夺似乎不是这些小鼠视网膜脱离中感光细胞保护的机制之一。
PURPOSE To study the neuroprotective effect of experimental retinal detachment (RD) on photoreceptor degeneration in rd1 mice. METHODS RD was produced in the eyes of rd1 mice at postnatal day (P) 9. These eyes were collected and compared to controls without RD. The effects of RD on retinal degeneration were evaluated by histochemical staining of nuclei in the outer nuclear layer (ONL), rod and cone photoreceptors, and retinal vessels at P30 in retinal sections and flatmounts. Apoptotic photoreceptors were detected by TdT-mediated dUTP nick-end labeling (TUNEL) at P15. Mice with or without RD were also reared in darkness and evaluated immunohistochemically at P30. RESULTS The numbers of rhodopsin-positive (rod), peanut agglutinin-positive (cone), and diamino-2-phenyl-indol-stained (rod-plus-cone) cells in the ONL were increased by 2.0-fold, 1.3-fold, and 1.2-fold, respectively, in the rd1 eyes with RD compared to those without RD at P30. In the detached retina, the cone photoreceptor inner/outer segment structures and the deep retinal vessels surrounding the inner nuclear layer and the ONL, but not the ganglion cell layer, were preserved. At P15, TUNEL-positive cell numbers in the ONL were significantly reduced in the eyes with RD. Light exposure had no effect on photoreceptor degeneration in the eyes with or without RD. CONCLUSIONS RD mediates the preservation of cone and rod photoreceptors in the ONL and surrounding vascular structures by reducing the rate of apoptosis of photoreceptors in rd1 mice. Light deprivation does not appear to be one of the mechanisms of photoreceptor protection in the detached retinas in these mice.