Substance P receptor regulates the photic induction of Fos-like protein in the suprachiasmatic nucleus of Syrian hamsters
Substance P receptor regulates the photic induction of Fos-like protein in the suprachiasmatic nucleus of Syrian hamsters
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DOI：
10.1016/0006-8993(95)01298-2
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发表时间：
1996-02-05
期刊：
影响因子：
2.9
通讯作者：
Honma, K
中科院分区：
文献类型：
--
作者：
Abe, H;Honma, S;Honma, K
P物质（Substance P，SP）是一种神经递质或神经调质，在哺乳动物中负责将光信息从视网膜传递到下丘脑视交叉上核（hypothalamic suprachiasmatic nucleus，SCN）。在主观夜间短暂的光照下，SCN中的即刻早期基因c-fos被诱导产生，并被认为在振荡器的光诱导中起重要作用。为了阐明SP受体参与SCN中c-fos光诱导的可能性，我们研究了SP受体拮抗剂Spantide对叙利亚仓鼠SCN中光诱导的Fos样蛋白免疫反应性（Fos-lir）的影响。Spantide预处理可抑制光诱导的Fos-lir，且呈剂量相关性和解剖学特征。较高剂量的spantide（8 nmol）阻断光诱导的Fos-lir基本上在嘴侧和中央地区的SCN，并在背侧部分的尾部SCN。然而，它仅在尾侧SCN的腹侧部分轻微地阻断Fos-lir。这些结果表明，SP参与传递光信息，诱导Fos蛋白在仓鼠SCN，不同的神经递质系统参与光诱导的Fos-lir在仓鼠SCN的不同部分。
Substance P (SP) is a candidate neurotransmitter or neuromodulator for conveying light information from the retina to the hypothalamic suprachiasmatic nucleus (SCN) where a circadian oscillator(s) is located in mammals. Immediate early gene c-fos has been demonstrated to be induced in the SCN with a brief light exposure at the subjective night, and suggested to play an important role in the photic entrainment of the oscillator. To clarify the possibility of an involvement of the SP receptor in the photic-induction of c-fos in the SCN, we examined effects of a SP receptor antagonist, spantide, on the light-induced Fos-like protein immunoreactivity (Fos-lir) in the SCN of Syrian hamster. The light-induced Fos-lir was inhibited with the pretreatment of spantide in a dose-related manner and in an anatomically distinctive way. The higher dose of spantide (8 nmol) blocked light-induced Fos-lir substantially in the rostral and central areas of the SCN, and in the dorsal portion of the caudal SCN. However, it blocked Fos-lir only slightly in the ventral portion of the caudal SCN. These results suggest that the SP is involved in conveying light information to induce Fos protein in the hamster SCN, and that different neurotransmitter systems are involved in the light-induced Fos-lir in the different portions of hamster SCN.