Bioanalysis and pharmacokinetics of chitosan ester in rabbit serum by HPLC with postcolumn fluorescence derivatization
Bioanalysis and pharmacokinetics of chitosan ester in rabbit serum by HPLC with postcolumn fluorescence derivatization
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DOI：
10.1016/j.jchromb.2006.07.058
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发表时间：
2007-01-01
影响因子：
3
通讯作者：
Wang, Yuanhong
中科院分区：
文献类型：
--
作者：
Han, Yuhong;Lv, Zhihua;Wang, Yuanhong
人们对壳聚糖酯 (PS916) 与源自海洋甲壳素的新型硫酸氨基多糖的抗动脉粥样硬化活性的兴趣，需要开发一种灵敏且特异的方法来研究其药代动力学。开发并验证了一种灵敏且可重复的柱后荧光衍生化高效液相色谱 (HPLC)，用于测定兔血清中的 PS916。使用C8反相柱进行色谱分析，流动相由甲醇-水(20:80，v/v)组成，流速为0.2ml/min。衍生化过程涉及在 110 摄氏度的碱性介质中与盐酸胍进行柱后反应。荧光检测器在 250 nm（激发）和 435 nm（发射）下操作。该测定在5-100μg/ml的浓度范围内呈线性。发现检测下限 (LLOD) 为 1.0 μg/ml。该方法成功应用于PS916在家兔体内的药代动力学研究。 (c) 2006 Elsevier B.V. 保留所有权利。
Interest in antiatherosclerotic activity of chitosan ester (PS916) with a new form of sulfate amino polysaccharide derived from marine chitin has necessitated the development of a sensitive and specific method to study its pharmacokinetics. A sensitive and reproducible high-performance liquid chromatography (HPLC) with postcolumn fluorescence derivatization method was developed and validated for the determination of PS916 in rabbit serum. Chromatography was carried out using a C8 reversed-phase column with an isocratic mobile phase consisting of methanol-water (20:80, v/v) at a flow rate of 0.2 ml/min. The derivatization procedure involved postcolumn reaction with guanidine hydrochloride in an alkaline medium at 110 degrees C. The fluorometric detector was operated at 250 nm (excitation) and 435 nm (emission). The assay was linear over the concentration range of 5-100 mu g/ml. The lower limit of detection (LLOD) was found to be 1.0 mu g/ml. The proposed method was successfully applied for a pharmacokinetic study of PS916 in rabbits. (c) 2006 Elsevier B.V. All rights reserved.