Surfing the wave of oxyfunctionalization chemistry by engineering fungal unspecific peroxygenases
Surfing the wave of oxyfunctionalization chemistry by engineering fungal unspecific peroxygenases
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DOI：
10.1016/j.sbi.2022.102342
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发表时间：
2022-02-28
影响因子：
6.8
通讯作者：
Alcalde, Miguel
中科院分区：
文献类型：
--
作者：
Beltran-Nogal, Alejandro;Sanchez-Moreno, Israel;Alcalde, Miguel
迄今为止，将氧选择性插入到未活化的有机分子中被认为对于合成现有的和下一代的工业化学品或药物是极其重要的。在这方面，真菌非特异性过氧化物酶（UPO）的最低要求和高活性使其成为C-H氧功能化生物催化剂皇冠上的宝石。虽然其有限的可用性和发展，阻碍了他们纳入工业，定向进化和计算设计的结合正在接近UPO的实际应用。在这篇综述中，我们将讨论UPO工程的最新进展，包括长UPO和短UPO系列，同时讨论这个快速发展的研究领域的未来前景。
The selective insertion of oxygen into non-activated organic molecules has to date been considered of utmost importance to synthesize existing and next generation industrial chemicals or pharmaceuticals. In this respect, the minimal requirements and high activity of fungal unspecific peroxygenases (UPOs) situate them as the jewel in the crown of C-H oxyfunctionalization biocatalysts. Although their limited availability and development has hindered their incorporation into industry, the conjunction of directed evolution and computational design is approaching UPOs to practical applications. In this review, we will address the most recent advances in UPO engineering, both of the long and short UPO families, while discussing the future prospects in this fast-moving field of research.