Hemokinin-1 mediates pruriceptive processing in the rat spinal cord.
Hemokinin-1 mediates pruriceptive processing in the rat spinal cord.
复制标题
Hemokinin-1 介导大鼠脊髓的痒觉处理。
DOI：
10.1016/j.neuroscience.2014.07.001
复制
发表时间：
2014
期刊：
影响因子：
3.3
通讯作者：
Y. Ishida
中科院分区：
文献类型：
--
作者：
H. Funahashi;R.Naono-Nakayama;K. Ebihara;G. Koganemaru;A. Kuramashi;T. Ikeda,T. Nishimori;Y. Ishida
血激肽-1（HK-1）是一种新的哺乳动物速激肽，其氨基酸序列与P物质（SP）相似。虽然SP，一种代表性的速激肽肽，在疼痛系统中的功能已经被很好地确定，但HK-1的功能尚未被阐明。[Leu 11]-SP对SP诱导的抓挠行为有拮抗作用，提示[Leu 11]-HK-1也可减弱HK-1诱导的抓挠行为。因此，评价了用[Leu 11]-HK-1预处理的效果以阐明HK-1的功能。鞘内注射[Leu 11]-HK-1可减弱HK-1对抓挠的诱导作用，但不能减弱SP对抓挠的诱导作用，而[Leu 11]-SP可减弱SP对抓挠的诱导作用，但不能减弱HK-1的作用。提示[Leu 11]-HK-1可能是HK-1首选受体的特异性拮抗剂，[Leu 11]-SP对SP首选受体--神经激肽1受体有拮抗作用。在福尔马林试验中，[Leu 11]-SP鞘内给药，但不是[Leu 11]-HK-1，减少福尔马林注射到后爪的足底区域后脊髓背角的退缩和c-Fos阳性细胞的数量。这些结果表明，SP，而不是HK-1，参与在脊髓水平的伤害性加工。为了评估HK-1和SP在瘙痒处理中的参与，评价了[Leu 11]-HK-1和[Leu 11]-SP对5-羟色胺（5-HT）和组胺诱导的抓挠行为和c-Fos表达的影响。[Leu 11]-HK-1和[Leu 11]-SP预处理可明显减弱5-HT和组胺诱导的抓挠行为和c-Fos表达的增加，提示HK-1和SP可能参与了抓挠加工。这些结果表明，HK-1参与了钙钛矿的加工过程，[Leu 11]-HK-1是阐明钙钛矿加工机制的一个有价值的工具。2014 IBRO。由爱思唯尔有限公司出版。保留所有权利。
Hemokinin-1 (HK-1) is a new mammalian tachykinin peptide consisting of the amino acid sequence similar to substance P (SP). Although the function of SP, a representative tachykinin peptide, has been well established in the pain system, that of HK-1 has not yet been elucidated.[Leu11]-SP had an antagonistic effect on SP-induced scratching behavior, suggesting that [Leu11]-HK-1 may also attenuate the induction of scratching behavior by HK-1. Thus, the effects of a pretreatment with [Leu11]-HK-1 were evaluated to clarify the function of HK-1. The intrathecal administration of [Leu11]-HK-1 attenuated the induction of scratching by HK-1, but not SP, while [Leu11]-SP reduced the induction of scratching by SP, but not HK-1. These results indicated that [Leu11]-HK-1 may be a more specific antagonist of HK-1-preferred receptors and [Leu11]-SP has an antagonistic effect on the SP-preferred receptor, the neurokinin 1 receptor. In the formalin test for examining noxious response, the intrathecal administration of [Leu11]-SP, but not [Leu11]-HK-1, reduced the number of flinchings and c-Fos-positive cells in the spinal dorsal horn following formalin injection into the plantar region of the hind paw. These results indicated that SP, but not HK-1, is involved in nociceptive processing at the spinal level. To evaluate the involvement of HK-1 and SP in pruritic processing, the effect of [Leu11]-HK-1 and [Leu11]-SP on the induction of scratching behavior and c-Fos expression by serotonin (5-HT) and histamine was evaluated. The increased induction of scratching behavior and c-Fos expression by 5-HT and histamine was markedly attenuated by pretreatment with both [Leu11]-HK-1 and [Leu11]-SP, suggesting that HK-1 and SP may be involved in pruritic processing. These results indicate that HK-1 is involved in pruritic processing and [Leu11]-HK-1 is a valuable tool for clarifying the mechanisms underlying pruritic processing. Ó 2014 IBRO. Published by Elsevier Ltd. All rights reserved.