Multimodal silica-shelled quantum dots: Direct intracellular delivery, photosensitization, toxic, and microcirculation effects
Multimodal silica-shelled quantum dots: Direct intracellular delivery, photosensitization, toxic, and microcirculation effects
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DOI：
10.1021/bc700431c
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发表时间：
2008-06-01
影响因子：
4.7
通讯作者：
Kanno, Iwao
中科院分区：
文献类型：
--
作者：
Bakalova, Rumiana;Zhelev, Zhivko;Kanno, Iwao
在本研究中，我们描述了一种多模式量子点探针与组合的荧光和顺磁特性，基于二氧化硅壳的单量子点胶束与纳入顺磁物质[三（2，2，6，6-四甲基-3，5-庚二酸盐）/钆]到胶束和/或二氧化硅涂层。该探针的特点是具有高的光致发光量子产率和良好的阳性MRI对比度，低细胞毒性，并易于在活细胞内递送。在实验动物中静脉内施用探针并不显著影响生理参数和微循环（例如，心率、血压、血管的直径和形状），这使得它适用于使用荧光共聚焦显微镜、双光子显微镜和MRI跟踪血液循环和体内多模态成像。
In the present study, we describe a multimodal QD probe with combined fluorescent and paramagnetic properties, based on silica-shelled single QD micelles with incorporated paramagnetic substances [tris(2,2,6,6-tetramethyl-3,5-heptanedionate)/gadolinium] into the micelle and/or silica coat. The probe was characterized with high photoluminescence quantum yield and good positive MRI contrast, low cytotoxicity, and easy intracellular delivery in viable cells. The intravenous administration of the probe in experimental animals did not affect significantly the physiological parameters and microcirculation (e.g., heart rate, blood pressure, diameter and shape of blood vessels), which makes it appropriate for tracing of blood circulation and in vivo multimodal imaging using fluorescent confocal microscopy, two-photon microscopy, and MRI.