Two-photon imaging of capillary blood flow in olfactory bulb glomeruli
Two-photon imaging of capillary blood flow in olfactory bulb glomeruli
复制标题
DOI：
10.1073/pnas.2133652100
复制
发表时间：
2003-10-28
影响因子：
11.1
通讯作者：
Charpak, S
中科院分区：
文献类型：
--
作者：
Chaigneau, E;Oheim, M;Charpak, S
分析神经元活动和脑血流之间的时空耦合需要精确测量单个毛细血管中红细胞流动的动态，这些毛细血管灌溉激活的神经元。在这里，我们使用双光子显微镜在体内对大鼠背嗅球肾小球毛细血管中的单个红细胞进行成像。我们发现气味刺激引起的毛细血管反应是气味和肾小球特异性的。这些反应包括红细胞流量的增加和减少，两者都是由红细胞速度或线性密度的独立变化引起的。最后，利用微米级空间分辨率和毫秒级时间分辨率测量红细胞流动，我们证明，在嗅球浅层，毛细血管反应精确地勾勒出突触激活区域。
Analysis of the spatiotemporal coupling between neuronal activity and cerebral blood flow requires the precise measurement of the dynamics of RBC flow in individual capillaries that irrigate activated neurons. Here, we use two-photon microscopy in vivo to image individual RBCs in glomerular capillaries in the rat dorsal olfactory bulb. We find that odor stimulation evokes capillary vascular responses that are odorant- and glomerulus-specific. These responses consist of increases as well as decreases in RBC flow, both resulting from independent changes in RBC velocity or linear density. Finally, measuring RBC flow with micrometer spatial resolution and millisecond temporal resolution, we demonstrate that, in olfactory bulb superficial layers, capillary vascular responses precisely outline regions of synaptic activation.