Pallenopsis patagonica (Hoek, 1881) - a species complex revealed by morphology and DNA barcoding, with description of a new species of Pallenopsis Wilson, 1881
Pallenopsis patagonica (Hoek, 1881) - a species complex revealed by morphology and DNA barcoding, with description of a new species of Pallenopsis Wilson, 1881
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DOI：
10.1111/zoj.12097
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发表时间：
2014
影响因子：
2.8
通讯作者：
A. Weis;R. Meyer;Lars Dietz;Jana S. Dömel;F. Leese;R. Melzer
中科院分区：
文献类型：
--
作者：
A. Weis;R. Meyer;Lars Dietz;Jana S. Dömel;F. Leese;R. Melzer
Pallenopsis patgon ica(Hoek，1881)是最具分类学问题和变异性最大的雀科植物之一，分布于南美洲南部海岸、亚南极和南极地区。对47个蝴蝶兰标本的线粒体细胞色素C氧化酶亚基I(COI)序列进行了系统发育分析，其中39个标本经形态鉴定为Pallenopsis patagica，5个Pallenopsis Pilosa(Hoek，1881)，1个Pallenopsis macneilli Clark，1963，1个Pallenopsis buphtalmus Pushkin，1个Pallenopsis latefrontalis Pushkin，1993。此外，我们还利用光学和扫描电子显微镜研究了不同COI谱系之间的形态差异，包括来自Loman和Hedgpeth经典收藏的材料，以及1881年以来Hoek的类型材料。分子水平的研究结果明确地表明，帕塔哥尼卡原虫是一个由多个分支组成的复合体，其中还包括马氏原甲藻、布氏原甲藻和短冠原甲藻。根据现有的材料，可以识别出两个主要的支系，即一个‘福克兰’支系和一个‘智利’支系，前者被我们命名为名义上的帕塔哥尼察，后者有别于‘福克兰’支系。我们将‘智利’支系描述为新种--叶海兰Pallenopsis yepayekae sp.11月韦斯，2013年。所有的分子结果都被详细讨论的特殊形态特征所证实，并与与帕塔哥尼察复合体密切相关的Pallenopsis种进行了比较。我们的结果表明，帕塔哥尼卡是一个物种丰富的复合体，需要用形态和遗传学的方法进行彻底的分类修订。©2014伦敦林尼安协会
Pallenopsis patagonica (Hoek, 1881) is one of the most taxonomically problematic and variable pycnogonid species, and is distributed around the southern South American coast, and the Subantarctic and Antarctic areas. We conducted a phylogenetic analysis of mitochondrial cytochrome c oxidase subunit I (COI) sequences of 47 Pallenopsis specimens, including 39 morphologically identified as P. patagonica, five Pallenopsis pilosa (Hoek, 1881), one Pallenopsis macneilli Clark, 1963, one Pallenopsis buphtalmus Pushkin, 1993, and one Pallenopsis latefrontalis Pushkin, 1993. Furthermore, we studied morphological differences between the different COI lineages using light and scanning electron microscopy, including also material from Loman's and Hedgpeth's classical collections, as well as Hoek's type material of P. patagonica from 1881. The molecular results unambiguously reveal that P. patagonica is a complex of several divergent clades, which also includes P. macneilli, P. buphtalmus, and P. latefrontalis. Based on the material available, two major clades could be identified, namely a ‘Falkland’ clade, to which we assign the nominal P. patagonica, and a ‘Chilean’ clade, which is distinct from the ‘Falkland’ clade. We describe the ‘Chilean’ clade as new species, Pallenopsis yepayekae sp. nov. Weis, 2013. All molecular results are confirmed by specific morphological characteristics that are discussed in detail and compared with Pallenopsis species closely related to the P. patagonica complex. Our results reveal that P. patagonica is a species-rich complex that is in need for a thorough taxonomic revision, using both morphological and genetic approaches. © 2014 The Linnean Society of London