1H NMR analysis of the lactose/β-galactosidase-derived galacto-oligosaccharide components of Vivinal® GOS up to DP5
1H NMR analysis of the lactose/β-galactosidase-derived galacto-oligosaccharide components of Vivinal® GOS up to DP5
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DOI：
10.1016/j.carres.2014.08.012
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发表时间：
2014-12-05
影响因子：
3.1
通讯作者：
Kamerling, Johannis P.
中科院分区：
文献类型：
--
作者：
van Leeuwen, Sander S.;Kuipers, Bas J. H.;Kamerling, Johannis P.
Vivinal (R) GOS 是一种低聚半乳糖 (GOS) 产品，由乳糖与环状芽孢杆菌 β-半乳糖苷酶 (EC 3.2.1.23) 一起孵育而制备。这种聚合度 (DP) 为 1 至 8 的复杂糖类混合物通常应用于婴儿营养品中。这里给出了达到DP5级别的商业产品的详细结构描述。首先，使用HPLC-SEC（Rezex RSO-01寡糖Ag+柱）对Vivinal (R) GOS进行DP分析和H-1 NMR分析。然后，将产物在 Bio-Gel P-2 上分级，并根据 DP 合并获得的级分，如 MALDI-TOF-MS 分析所示。最后，通过 1D/2D H-1/C-13 NMR 光谱和键合分析对单一 DP 的级分及其通过 CarboPac PA-1 上的 HPAEC-PAD 获得的亚级分进行了分析。总共对 40 多个结构进行了表征，提供了超过 99% 的产品结构覆盖率。详细的 H-1 和 C-13 NMR 数据，以及 G.U.包括所有寡糖的 CarboPac PA-1 上的值（葡萄糖单位；麦芽寡糖梯）。 (C) 2014 Elsevier Ltd. 保留所有权利。
Vivinal (R) GOS is a galacto-oligosaccharide (GOS) product, prepared from lactose by incubation with Bacillus circulans beta-galactosidase (EC 3.2.1.23). This complex mixture of saccharides with degree of polymerization (DP) between 1 and 8 is generally applied in infant nutrition. Here, a detailed structural description of the commercial product up to the DP5 level is given. First, Vivinal (R) GOS was subjected to DP analysis using HPLC-SEC (Rezex RSO-01 oligosaccharide Ag+ column) and H-1 NMR analysis. Then, the product was fractionated on Bio-Gel P-2, and the obtained fractions were pooled according to DP, as indicated by MALDI-TOF-MS analysis. Finally, fractions of single DP, as well as their subfractions obtained by HPAEC-PAD on CarboPac PA-1, were analyzed by 1D/2D H-1/C-13 NMR spectroscopy and linkage analysis. In total, over 40 structures, providing a structural coverage of over 99% of the product, have been characterized. Detailed H-1 and C-13 NMR data, as well as G.U. values (glucose units; malto-oligosaccharide ladder) on CarboPac PA-1 of all oligosaccharides are included. (C) 2014 Elsevier Ltd. All rights reserved.