Kappa-squared: from nuisance to new sense.
Kappa-squared: from nuisance to new sense.
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DOI：
10.1016/s1389-0352(01)00037-x
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发表时间：
2002-01-01
影响因子：
4.1
通讯作者：
van der Meer, B W
中科院分区：
文献类型：
--
作者：
van der Meer, B W
通常称为kappa-squared的取向因子通常被认为是一个麻烦，因为它代表了用FRET技术获得的距离的显著不确定性。结果表明，这种不确定性是相当小的，在几乎所有的情况下，实际利益，如果一个采取的宽度为67%的置信区间（CI）的距离分布作为一个衡量的不确定性。卡帕平方有可能开辟新的信息的方向和旋转的时间分辨研究的供体和受体的各向异性。人们可以通过设计描述系统中施主和受主的各种取向和位置的状态之间的转换的矩阵模型来理解这些数据。
The orientation factor, which is commonly called kappa-squared, is often considered to be a nuisance because it represents a significant uncertainty in the distance obtained with the FRET technique. It is shown that this uncertainty is rather small in almost all cases of practical interest if one takes the width of a 67% confidence interval (CI) for the distance distribution as a measure of uncertainty. Kappa-squared has the potential to open up new information on orientations and rotations from time-resolved studies of donor and acceptor anisotropies. One can make sense of such data by designing matrix models for the transitions between states describing various orientations and positions of donors and acceptors in the system.