Anxiolytic effects of polydatin through the blockade of neuroinflammation in a chronic pain mouse model
Anxiolytic effects of polydatin through the blockade of neuroinflammation in a chronic pain mouse model
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虎杖甙通过阻断慢性疼痛小鼠模型的神经炎症发挥抗焦虑作用
DOI：
10.1177/1744806919900717
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发表时间：
2020-01-01
期刊：
影响因子：
3.3
通讯作者：
Zhao, Ming-Gao
中科院分区：
文献类型：
--
作者：
Guan, Shao-Yu;Zhang, Kun;Zhao, Ming-Gao
背景：慢性疼痛常与焦虑障碍合并症，从而使其治疗复杂化。虎杖苷是虎杖根的一种成分，对中枢神经系统具有保护作用。然而，它对疼痛和焦虑处理的影响很少被研究。在本研究中，小鼠后爪注射完全的弗氏佐剂（CFA）来诱导疼痛和焦虑样行为。结果25 mg/kg多聚丹素可减轻小鼠的焦虑样行为，但对疼痛感觉无明显影响。多果苷处理逆转了注射cfa小鼠杏仁核中n -甲基- d -天冬氨酸受体和含glua1 α-氨基-3-羟基-5-甲基-4-异恶唑-丙酸受体的上调。此外，这种治疗降低了扁桃体中促炎细胞因子的水平，即肿瘤坏死因子- α和白细胞介素-1β。此外，激活的核因子kappa-B信号在cfa注射小鼠的杏仁核中被阻断。通过对接技术，我们发现多聚糖与i - κ b激酶β之间存在潜在的结构结合。结论多柚素通过抑制杏仁核炎症因子发挥抗焦虑作用。
Background Chronic pain is frequently comorbid with anxiety disorder, thereby complicating its treatment. Polydatin, a component from the root of Polygonum cuspidatum, has shown neuroprotection in the central nervous system. However, its effects on pain and anxiety processing have been rarely investigated. In this study, mice were injected with complete Freund’s adjuvant (CFA) at the hindpaw to induce pain- and anxiety-like behaviors. Results Treatment with polydatin (25 mg/kg) alleviated the anxiety-like behaviors but not pain perception in these mice. Polydatin treatment reversed the upregulation of N-methyl-D-aspartic acid receptors and GluA1-containing α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptors in the amygdala of CFA-injected mice. Additionally, this treatment reduced the levels of proinflammatory cytokines, namely, tumor necrosis factor-alpha and interleukin-1β, in the amygdala. Furthermore, activated nuclear factor kappa-B signaling was blocked in the amygdala from CFA-injected mice. By using docking technology, we found potential structural binding between polydatin and IκB kinase beta. Conclusion This study indicates the anxiolytic effects of polydatin by suppressing inflammatory cytokines in the amygdala.