A role for the deep orange and carnation eye color genes in lysosomal delivery in Drosophila
A role for the deep orange and carnation eye color genes in lysosomal delivery in Drosophila
复制标题
DOI：
10.1016/s1097-2765(00)80199-9
复制
发表时间：
1999-10-01
期刊：
影响因子：
16
通讯作者：
Krämer, H
中科院分区：
文献类型：
--
作者：
Sevrioukov, EA;He, JP;Krämer, H
深橙子和康乃馨是果蝇中两个经典的眼睛颜色基因，在这里，我们证明了深橙子是定位于内体区室的蛋白质复合物的一部分。这个复合物的第二个组成部分是康乃馨，一种类似Sec1p的膜融合调节因子的同系物。由于完全丧失深橙子功能是致命的，因此在深橙子突变体克隆中分析了该复合物在细胞内运输中的作用。缺乏深橙子功能的视网膜细胞完全缺乏色素沉着，并表现出夸张的多泡结构，此外，在发育中的感光细胞中可见内吞运输缺陷。这些结果提供了直接的证据表明，眼睛颜色突变的颗粒组也破坏囊泡运输到溶酶体。
deep orange and carnation are two of the classic eye color genes in Drosophila, Here, we demonstrate that Deep orange is part of a protein complex that localizes to endosomal compartments. A second component of this complex is Carnation, a homolog of Sec1p-like regulators of membrane fusion. Because complete loss of deep orange function is lethal, the role of this complex in intracellular trafficking was analyzed in deep orange mutant clones. Retinal cells devoid of deep orange function completely lacked pigmentation and exhibited exaggerated multivesicular structures, Furthermore, a defect in endocytic trafficking was visualized in developing photoreceptor cells. These results provide direct evidence that eye color mutations of the granule group also disrupt vesicular trafficking to lysosomes.