Ethanol extract of Patrinia scabiosaefolia induces the death of human renal cell carcinoma 786-O cells via SIRT-1 and mTOR signaling-mediated metabolic disruptions
Ethanol extract of Patrinia scabiosaefolia induces the death of human renal cell carcinoma 786-O cells via SIRT-1 and mTOR signaling-mediated metabolic disruptions
复制标题
败酱乙醇提取物通过 SIRT-1 和 mTOR 信号介导的代谢破坏诱导人肾细胞癌 786-O 细胞死亡
DOI：
10.3892/or.2017.6139
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发表时间：
2018-02-01
期刊：
影响因子：
4.2
通讯作者：
Zhang, Wenhua
中科院分区：
文献类型：
--
作者：
Li, Zeyan;Tang, Yueqing;Zhang, Wenhua
近年来，天然植物提取物作为新型抗肿瘤药物显示出巨大的潜力。黄芩是治疗炎症性疾病的传统处方，据报道可以有效抑制各种类型的癌症。然而，其抗肿瘤特性的机制尚不清楚。本实验研究了黄腐草（Patrinia scabiosaefolia， EPS）乙醇提取物对人肾癌786-O细胞的抗肿瘤作用。与EPS孵育24 h后，通过MTT和菌落形成实验，786-O细胞的细胞活力和菌落数与对照组相比呈浓度依赖性显著降低。LDH释放法和Hoechst 33342/PI双染色法显示，EPS暴露组细胞坏死率和凋亡率均显著高于对照组。在浓度为1.0 mg/ml时，细胞坏死率为41.7 +/- 6.6，凋亡率为7.8 +/- 1.4% (P
Recently, natural plant extracts have shown tremendous potential as novel antitumor drugs. Patrinia scabiosaefolia, a traditional prescription for inflammatory diseases, has been reported to effectively suppress various types of cancers. However, the mechanisms underlying its antitumor properties remain elusive. In the present study, we investigated the antitumor effects of an ethanol extract of Patrinia scabiosaefolia (EPS) on human renal cell carcinoma 786-O cells. After 24 h of incubation with EPS, the cell viability and colony number of 786-O cells were significantly decreased in a concentration-dependent manner as compared to the control group as determined by MTT and colony formation assays, respectively. The necrotic rate and apoptotic rate in the EPS exposure group were significantly higher than these rates noted in the control group as revealed by LDH release assay and Hoechst 33342/PI double staining, respectively. At the concentration of 1.0 mg/ml, the necrotic and apoptotic rates reached 41.7 +/- 6.6 and 7.8 +/- 1.4%, respectively (P