Factors affecting the in vitro action of cumulus cells on the maturing mouse oocytes
Factors affecting the in vitro action of cumulus cells on the maturing mouse oocytes
复制标题
DOI：
10.1002/mrd.20753
复制
发表时间：
2008-01-01
影响因子：
2.5
通讯作者：
Tan, Jing-He
中科院分区：
文献类型：
--
作者：
Ge, Li;Han, Dong;Tan, Jing-He
在生殖囊泡 (GV) 阶段从卵母细胞中去除卵丘细胞 (CC) 仍然是 GV 移植、体细胞单倍化和卵母细胞冷冻保存等胚胎技术的主要限制。然而，在哺乳动物物种中尚未建立有效的 CC 剥脱卵母细胞 (DO) 体外成熟 (IVM) 系统。尽管卵泡细胞被认为在卵母细胞成熟中发挥重要作用，但不同细胞类型的具体作用和作用机制却知之甚少。关于 CC 与卵母细胞之间的连接关联是否对于 CC 共培养对卵母细胞成熟的有益作用至关重要的报道存在冲突。我们的目标是尝试使用小鼠卵母细胞模型来解决这些问题。结果表明，虽然与CC单层共培养只能部分恢复没有电晕细胞的DOs的发育潜力，但它完全恢复了电晕封闭的DOs的能力。在CC单层条件培养基中培养也促进了DO的成熟。然而，与单层壁颗粒细胞共培养没有效果。 CC 共培养的效率受到多种因素的影响，例如 CC 的密度和年龄、成熟培养基中促性腺激素的存在以及体内 (IVO) 促性腺激素引发的持续时间。结论是，小鼠 CC 产生一种可扩散因子，以 CC-卵母细胞连接通讯依赖的方式支持 DO 成熟。这些数据将有助于我们了解 CC 促进卵母细胞成熟的机制，并有助于建立有效的 DO IVM 系统。
The removal of cumulus cells (CCs) from oocytes at the germinal vesicle (GV) stage still represents a major limitation in such embryo techniques as GV transfer, somatic cell haploidization, and oocyte cryopreservation. However, no efficient in vitro maturation (IVM) system for CC-denuded oocytes (DOs) has been established in mammalian species. Although follicular cells are considered to play an important role in oocyte maturation, the specific role and mechanisms of action of different cell types are poorly understood. Reports on whether junctional association between CCs and the oocyte is essential for the beneficial effect of CC co-culture on oocyte maturation are in conflict. Our objective was to try to address these issues using the mouse oocyte model. The results indicated that while co-culture with the CC monolayer could only partially restore the developmental potential of DOs without corona cells, it restored the competence of corona-enclosed DOs completely. Culture in medium conditioned with CC monolayer also promoted maturation of DOs. However, co-culture with the monolayer of mural granulosa cells had no effect. The efficiency of CC co-culture was affected by various factors such as density and age of the CCs, the presence of gonadotropin in the maturation medium and the duration for in vivo (IVO) gonadotropin priming. It is concluded that mouse CCs produce a diffusible factor(s) that support DO maturation in a CC-oocyte junctional communication dependent manner. The data will contribute to our understanding the mechanisms by which CCs promote oocyte maturation and to the establishment of an efficient DO IVM system.