Biomimetic small exosome with outstanding surgical applications for rapid large-scale wound healing and functional sweat gland restoration
Biomimetic small exosome with outstanding surgical applications for rapid large-scale wound healing and functional sweat gland restoration
复制标题
仿生小外泌体具有出色的外科应用，可用于快速大规模伤口愈合和功能性汗腺恢复
DOI：
10.1016/j.nantod.2022.101531
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发表时间：
2022-08-01
期刊：
影响因子：
17.4
通讯作者：
Sun, Xiaoyan
中科院分区：
文献类型：
--
作者：
Chen, Huating;Liu, Yawei;Sun, Xiaoyan
真皮和表皮细胞类型的协调浸润的实现以及细胞外基质的快速沉积是改善大面积皮肤缺损愈合质量的先决条件。然而，目前使用的材料由于固有的差的组织相容性、低渗透性、差的机械性能和细胞毒性而限制用于伤口愈合。开发新的治疗剂是具有挑战性的，所述新的治疗剂使得能够及时和有效地伤口愈合，同时具有最小的副作用以避免慢性伤口、伤口粘连和大的瘢痕形成。在此，我们制造了富含转化生长因子β 1（TGF-β 1）的仿生外泌体（EM），用于大规模皮肤伤口的快速愈合。EM包封允许TGF-β 1的良好管理的剂量，以通过赋予表皮角质形成细胞迁移特征、增强其干细胞性质并导致加速的体内伤口再上皮化来有效地促进伤口愈合过程。特别是，EM可以快速恢复热损伤小鼠模型中的汗腺功能。我们的工作为提高大面积伤口患者的愈合速度和愈合质量提供了一种有前途的策略。(c)2022由Elsevier Ltd.出版
The realization of coordinated infiltration of dermal and epidermal cell types, and the rapid deposition of extracellular matrix are the prerequisites for improved healing quality of large skin defects. However, presently used materials are limited for wound healing due to the inherent poor histocompatibility, low permeability, poor mechanical property and cytotoxicity. It is challenging to develop new therapeutic agents that enable timely and efficient wound healing and meanwhile there are minimal side effects to avoid chronic wounds, wound adhesions, and large scarring. Herein, we fabricated biomimetic exosomes (EMs) that enriched transforming growth factor beta 1 (TGF-beta 1) for the rapid healing of large-scale cutaneous wounds. The EM encapsulation allowed well-managed dosing of the TGF-beta 1 to effectively promote the wound healing process by endowing epidermal keratinocytes with a migratory feature, enhancing their stem cell properties, and resulting in accelerated in vivo wound re-epithelization. Specifically, the EMs could fast restore the function of sweat glands in a thermally injured mice model. Our work provides a promising strategy for improving the healing speed and healing quality for patients with large wounds. (c) 2022 Published by Elsevier Ltd.