False Positive Responses of Campylobacter jejuni when Using the Chemical-In-Plug Chemotaxis Assay
False Positive Responses of Campylobacter jejuni when Using the Chemical-In-Plug Chemotaxis Assay
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DOI：
10.1292/jvms.10-0396
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发表时间：
2011-03-01
影响因子：
1.2
通讯作者：
Takai, Shinji
中科院分区：
文献类型：
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作者：
Kanungpean, Doungjit;Kakuda, Tsutomu;Takai, Shinji
据报道，空肠弯曲杆菌对岩藻糖、几种氨基酸和有机酸、粘蛋白和胆汁表现出趋化行为。空肠弯曲菌的趋化性主要使用化学插塞趋化性测定法进行研究。在本研究中，构建了非趋化突变体（cheY 突变体）和非运动突变体（flhA 突变体）并用作测定中的阴性对照。在两种突变体中都观察到含有多种氨基酸和有机酸的测试塞周围明显的积累区域。我们的结果表明，化学塞测定中空肠弯曲菌的阳性反应并不总是表明趋化性。
Campylobacter jejuni reportedly exhibits chemotactic behavior towards fucose, several amino acids and organic acids, mucin and bile. The chemotaxis of C. jejuni has mainly been studied using the chemical-in-plug chemotaxis assay. In this study, a nonchemotactic mutant (cheY mutant) and nonmotile mutant (flhA mutant) were constructed and used as negative controls in an assay. Apparent zones of accumulation around test plugs containing several amino acids and organic acids were observed with both of the mutants. Our results suggest that positive responses of C. jejuni in the chemical-in-plug assay are not always indicative of chemotaxis.