A 96-well microplate incorporating a replica molded microfluidic network integrated with photonic crystal biosensors for high throughput kinetic biomolecular interaction analysis
A 96-well microplate incorporating a replica molded microfluidic network integrated with photonic crystal biosensors for high throughput kinetic biomolecular interaction analysis
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DOI：
10.1039/b618584c
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发表时间：
2007-01-01
期刊：
影响因子：
6.1
通讯作者：
Cunningham, Brian T.
中科院分区：
文献类型：
--
作者：
Choi, Charles J.;Cunningham, Brian T.
一个nanoreplica模塑工艺已被用来生产聚合物微流体通道，集成的无标记光子晶体生物传感器作为通道的底面。多个流动通道并行聚集，使得成像检测仪器可以同时监测许多生物分子相互作用的结合动力学。在这项工作中，流动通道模式已适应96孔微量板格式，其中对于微量板的每12个元件行，单个孔用作11个流动通道的公共入口，这些流动通道连接到单独的微量板孔。威尔斯。向公共井施加气动压力或抽吸用于驱动向前或向后流动到通道。通过测量蛋白A与高、中、低亲和力IgG分子的动力学结合相互作用来证明该系统。该方法提供了一种用于最小化使生物传感器表面功能化所需的试剂体积的方法，同时保持与生物研究中最常用的微孔板测定流体处理方法的兼容性。
A nanoreplica molding process has been used to produce polymer microfluidic channels, with integrated label-free photonic crystal biosensors as the bottom surface of the channels. Multiple flow channels are gathered in parallel so that an imaging detection instrument may simultaneously monitor the binding kinetics of many biomolecular interactions. In this work, the flow channel pattern has been adapted to a 96-well microplate format in which, for each 12-element row of the microplate, a single well serves as a common access port for 11 flow channels that are connected to separate microplate wells. Application of pneumatic pressure or suction to the common well serves to drive forward or backward flow to the channels. The system is demonstrated by measuring the kinetic binding interaction of protein A with IgG molecules of high, medium, and low affinity. The approach offers a means for minimizing the volume of reagent required to functionalize the biosensor surface, while retaining compatibility with the microplate assay fluid-handling methods that are most commonly used in biological research.