Loss of Hugl-1 expression associates with lymph node metastasis in endometrial cancer.
Loss of Hugl-1 expression associates with lymph node metastasis in endometrial cancer.
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DOI：
10.3727/000000007783980855
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发表时间：
2007
影响因子：
3.1
通讯作者：
T. Tsuruga;S. Nakagawa;Michiko Watanabe;S. Takizawa;Y. Matsumoto;K. Nagasaka;K. Sone;H. Hiraike;Y. Miyamoto;O. Hiraike;T. Minaguchi;K. Oda;T. Yasugi;T. Yano;Y. Taketani
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文献类型：
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作者：
T. Tsuruga;S. Nakagawa;Michiko Watanabe;S. Takizawa;Y. Matsumoto;K. Nagasaka;K. Sone;H. Hiraike;Y. Miyamoto;O. Hiraike;T. Minaguchi;K. Oda;T. Yasugi;T. Yano;Y. Taketani
肿瘤抑制基因、潦草、圆盘大和致命的巨型幼虫（lgl）的突变会导致细胞极性破坏以及果蝇上皮细胞和神经母细胞的过度增殖。据报道，人类lgl同源物Hugl-1的表达减少与人类结肠癌和恶性黑色素瘤的发生和进展有关。为了探讨Hugl-1表达与子宫内膜癌临床特征之间的关联，我们检测了原发性子宫内膜癌组织中Hugl-1的表达。采用半定量逆转录聚合酶链反应（RT-PCR）检测86例原发性子宫内膜癌组织中Hugl-1 mRNA的表达。所有样本分为两组：Hugl-1 阳性和 Hugl-1 阴性。采用Fisher精确概率检验分析各组临床资料，采用Kaplan-Meier法和Log-rank检验比较各组生存率。 Hugl-1表达缺失与淋巴结转移的较高发生率相关，但与患者的发病年龄、远处转移、临床分期、淋巴或静脉血管侵犯或组织病理学分化级别无关。与Hugl-1阴性组相比，Hugl-1阳性组的预后较差。这些结果表明子宫内膜癌中Hugl-1表达的丧失可能导致淋巴结转移，并且可能是预后不良的一个因素。
Mutation of neoplastic tumor suppressor genes, scribble, discs large, and lethal giant larvae (lgl), causes disruption of cell polarity and overproliferation of Drosophila epithelial cells and neuroblasts. Reduced expression of human homologue of lgl, Hugl-1, has been reported to be involved in development and progression of human colon cancer and malignant melanoma. To explore the association between Hugl-1 expression and clinical character in endometrial cancer, we examined the expression of Hugl-1 in primary endometrial cancer tissues. The expression of Hugl-1 mRNA in 86 primary endometrial cancer tissues was examined using semiquantitative reverse transcription polymerase chain reaction (RT-PCR). All samples were categorized into two groups: Hugl-1 positive and Hugl-1 negative. Clinical data of each group were analyzed by Fisher's exact probability test and survival rates of each group were compared by Kaplan-Meier method and Log-rank test. Loss of Hugl-1 expression had correlation with the higher incidence of lymph node metastasis, but not to the patient's age at onset, distant metastasis, clinical stage, lymph or venous vessel invasion, or histopathological grade of differentiation. The Hugl-1-positive group had poorer prognosis compared with the Hugl-1-negative group. These results indicate that loss of Hugl-1 expression in endometrial cancer may contribute to lymph node metastasis and it can be a factor of poor prognosis.