Expression pattern of CXCR3, CXCR4, and CCR3 chemokine receptors in the developing human brain
Expression pattern of CXCR3, CXCR4, and CCR3 chemokine receptors in the developing human brain
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DOI：
10.1093/jnen/60.1.25
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发表时间：
2001-01-01
影响因子：
3.2
通讯作者：
Lavi, E
中科院分区：
文献类型：
--
作者：
Van der Meer, P;Goldberg, SH;Lavi, E
趋化因子受体是免疫和中枢神经系统的重要组成部分，但对其在发育过程中的分布知之甚少。我们评估了3种趋化因子受体：CXCR3、CXCR4和CCR3在人类发育中的大脑分布。其中，CXCR3是妊娠26周胎儿大脑中唯一表达的受体，其表达仅限于神经胶质细胞、内皮细胞和脉络膜丛。小脑浦肯野细胞仅在足月时可见神经元染色。CCR3仅在足月出现在神经元和神经胶质细胞中。这两种受体在妊娠后期和足月的表达模式与成虫相似。在胎儿大脑的神经元和神经胶质细胞上均未检测到CXCR4。通过检查儿科病例，我们确定CXCR4表达有时在3.5至4.5岁之间开始。两种趋化因子受体被检查。CCR3和CXCR3可与CD4一起作为HIV进入的共受体，但在妊娠中期均未表达。我们的研究结果表明，CCR3或CXCR4不太可能在妊娠37周前胎儿大脑中HIV-1传播中起主要作用。
Chemokine receptors are essential components of the immune and central nervous systems, but little is known about their distribution during development. We evaluated the distribution of 3 chemokine receptors: CXCR3, CXCR4, and CCR3 in the human developing brain. Of these, CXCR3 was the only receptor expressed in fetal brain at 26 wk of gestation and its expression was restricted to glial cells, endothelial cells, and the choroid plexus. Neuronal staining was only seen at term in the Purkinje cells of the cerebellum. CCR3 appeared only at term in both neurons and glial cells. The expression pattern of these 2 receptors in the late gestation and term resembled that of adults. CXCR4 could not be detected in the fetal brain on neurons nor on glial cells. By examining pediatric cases, we determined that CXCR4 expression commences sometimes between 3.5 and 4.5 yr. Two of the chemokine receptors examined. CCR3 and CXCR3, can be used as co-receptor together with CD4 for HIV entry, but neither was expressed during the second trimester of pregnancy. Our findings suggest that it is unlikely that CCR3 or CXCR4 play a major role in HIV-1 transmission in the fetal brain before 37 wk of gestation.