Molecular epidemiology of Paenibacillus larvae infection in Finland
Molecular epidemiology of Paenibacillus larvae infection in Finland
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DOI：
10.1051/apido:2008061
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发表时间：
2009-01-01
期刊：
影响因子：
2.4
通讯作者：
Pelkonen, Sinikka
中科院分区：
文献类型：
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作者：
Pentikainen, Jaana;Kalliainen, Eija;Pelkonen, Sinikka
在芬兰地区和养蜂场暂时研究了引起美国幼虫腐臭病（AFB）的类芽孢杆菌幼虫感染的流行病学。共156 P.幼虫分离物提供的蜂蜜或育雏样品114养蜂人进行了分析NotI脉冲场凝胶电泳（PFGE）和生物分型，以及四环素耐药性。检测到52个NotI宏限制性内切酶谱（MRP）和3个生物型。所有分离株均对四环素敏感。大多数MRP和生物型IV和V广泛分布在全国各地，尽管少数MRP和生物型I聚集在某些地区。随后从25个养蜂人的分离株的分析通常表明，在养蜂场感染的持续性，但在6例无关的感染出现。对来自一个养蜂人的当代分离株进行分型，发现在遥远的养蜂场存在相同的感染，从而表明感染的传播是通过养蜂实践而不是邻里。NotI PFGE能有效区分菌株，可推荐用于AFB的流行病学监测。
The epidemiology of a Paenibacillus larvae infection, causing American Foulbrood (AFB), was studied regionally in Finland and temporally in the apiaries. Altogether 156 P. larvae isolates from honey or brood samples supplied by 114 beekeepers were analysed by NotI pulsed-field gel electrophoresis (PFGE) and biotyping, as well as for tetracycline resistance. Fifty-two NotI macrorestriction profiles (MRP) and three biotypes were detected. All isolates were susceptible to tetracycline. Most MRPs and biotypes IV and V were widely spread throughout the country, though a few MRPs and biotype I were clustered around certain areas. Analysis of subsequent isolates from 25 beekeepers usually showed persistence of the infection in the apiaries, but in six cases unrelated infections emerged. Typing of contemporary isolates from one beekeeper revealed the presence of the same infection in distant apiaries, thus indicating that the spread of infection was through apicultural practices instead of neighbourhood. NotI PFGE allowed efficient strain discrimination and can be recommended for epidemiological surveillance of AFB.