Light-induced oxygen uptake in tobacco chloroplasts explained in terms of chlororespiratory activity
Light-induced oxygen uptake in tobacco chloroplasts explained in terms of chlororespiratory activity
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用氯呼吸活动解释烟草叶绿体光诱导的摄氧量
DOI：
10.1016/0005-2728(94)90053-1
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
G. Schmid
中科院分区：
文献类型：
--
作者：
W. Gruszecki;K. Bader;G. Schmid
高等植物（Nicotiana tabacum var. John William's Broadleaf）在用一个或多个短的饱和闪光照射时表现出大量的氧摄取。在缓冲反应测定中，将5 ml 18 O2加入气相后，通过质谱法检测并分析该摄取，作为18 O2-气体交换。沿着光诱导的氧吸收，我们测量了光合水氧化作为在m e= 32的氧释放。在m e= 36处测得的18 O2的氧摄取可被各种电子受体，特别是硅钼酸盐完全抑制。PS II抑制剂如DBMIB也是如此。讨论了DCMU和不同光质（蓝光、红光和远红光）对光诱导氧摄取的影响。我们得出结论，从我们的研究结果，类囊体膜内的chloropressive活性是负责所观察到的氧的吸收和质体醌池是两个，光合和呼吸电子传递链之间共享的组件。
Chloroplasts from higher plants (Nicotiana tabacum var. John William's Broadleaf) exhibit a substantial oxygen uptake upon illumination with one or more short saturating light flashes. This uptake was detected and analyzed by means of mass spectrometry as 18 O 2-gas exchange following the addition of 5 ml 18 O 2 to the gas phase over the buffered reaction assay. Along with the light-induced oxygen uptake we measured photosynthetic water oxidation as oxygen evolution at m e= 32. The oxygen uptake of 18 O 2 measured at m e= 36 can be completely inhibited by various electron acceptors and by silicomolybdate in particular. The same holds true for PS II-inhibitors like DBMIB. The effects of DCMU and of different light qualities (blue, red and far-red) on the light-induced oxygen uptake are discussed. We conclude from our results that the chlororespiratory activity within the thylakoid membranes is responsible for the observed oxygen uptake and that the plastoquinone pool is the component shared between both, photosynthetic and respiratory electron transport chains.