The effects of in vitro lipid peroxidation on the activity of rat liver microsomal glutathione S-transferase from rats supplemented or deficient in antioxidants.
The effects of in vitro lipid peroxidation on the activity of rat liver microsomal glutathione S-transferase from rats supplemented or deficient in antioxidants.
复制标题
体外脂质过氧化对补充或缺乏抗氧化剂的大鼠肝微粒体谷胱甘肽S-转移酶活性的影响。
DOI：
10.1016/0024-3205(88)90079-3
复制
发表时间：
1988
期刊：
影响因子：
6.1
通讯作者：
Stone,WL
中科院分区：
文献类型：
--
作者：
Harris,CM;Stone,WL
谷胱甘肽S-转移酶是一组多功能同工酶，在具有亲电中心的疏水性异生物质的解毒中发挥核心作用（1）。在这项研究中，我们研究了体外脂质过氧化作用对大鼠肝微粒体谷胱甘肽S-转移酶活性的影响，无论是补充或缺乏维生素E和硒。脂质过氧化反应的副产物丙二醛（MDA）的形成增加与大鼠肝微粒体谷胱甘肽S-转移酶活性降低有关。谷胱甘肽S-转移酶的抑制作用在喂食维生素E和硒缺乏饮食（B饮食）的大鼠的微粒体中迅速发生，但在喂食相同饮食但补充了这些微量营养素（B+E+Se饮食）的大鼠的微粒体中延迟15分钟。脂质过氧化抑制微粒体谷胱甘肽S-转移酶，这种抑制作用是由膳食抗氧化剂调节。
Glutathione S-transferases are a group of multifunctional isozymes that play a central role in the detoxification of hydrophobic xenobiotics with electrophilic centers (1). In this study we investigated the effects of in vitro lipid peroxidation on the activity of liver microsomal glutathione S-transferases from rats either supplemented or deficient in both vitamin E and selenium. Increased formation of malondialdehyde (MDA), a by-product of lipid peroxidation, was associated with a decreased activity of rat liver microsomal glutathione S-transferase. The inhibition of glutathione S-transferase occurred rapidly in microsomes from rats fed a diet deficient in both vitamin E and selenium (the B diet) but was delayed for 15 minutes in microsomes from rats fed the same diet but supplemented with these micro-nutrients (B+E+Se diet). Lipid peroxidation inhibits microsomal glutathione S-transferase and this inhibition is modulatted by dietary antioxidants.