Formation of odontoblast-like cells from cultured human dental pulp cells on dentin in vitro
Formation of odontoblast-like cells from cultured human dental pulp cells on dentin in vitro
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DOI：
10.1016/j.joen.2006.05.009
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发表时间：
2006-11-01
影响因子：
4.2
通讯作者：
Chan, Selina W.
中科院分区：
文献类型：
--
作者：
Huang, George T-J.;Shagramanova, Kristina;Chan, Selina W.
最近对人牙髓干细胞的鉴定为理解成牙本质细胞谱系提供了新的线索。本研究的目的是对体外分离和培养的成人牙髓细胞进行鉴定，并检测其在牙本质上的分化潜能。我们观察到，用酶消化法分离的一些牙髓细胞的增殖速度与永生细胞系NIH3T3相似。第3代牙髓细胞群体倍增时间(PDT)为22.6±-0.5h，NIH3T3为23.1+/-2.3h。牙髓细胞在地塞米松或地塞米松加1，25-二羟基维生素D3的刺激下形成矿物质结节。牙髓细胞接种到机械和化学处理的牙本质表面后，扫描电子显微镜分析显示，牙髓细胞似乎建立了成牙本质细胞样的形态，细胞质突起延伸到牙本质小管中。我们的研究结果表明，在现有的牙本质上可以形成具有成牙本质细胞形态的细胞，这表明分离的人牙髓干细胞在体外可以在牙本质上分化为成牙本质细胞。
Recent characterization of human dental pulp stem cells has shed new light on the understanding of the odontoblastic lineage. The purpose of the study was to characterize human adult dental pulp cells isolated and cultured in vitro and to examine the cell differentiation potential grown on dentin. We observed that some pulp cells isolated with an enzyme-digestion approach proliferated at a similar rate as the immortal cell line NIH 3T3. Population doubling time (PDt) for pulp cells at passage 3 was 22.6 +/- 0.5 hours and for NIH 3T3 was 23.1 +/- 2.3 hours. The pulp cells formed mineral nodules stimulated with dexamethasone or dexamethasone plus 1,25-dihydroxyvitamin D3. Pulp cells, after being seeded onto mechanically and chemically treated dentin surface, appeared to establish an odontoblast-like morphology with a cytoplasmic process extending into a dentinal tubule revealed by scanning electron microscopy analysis. Our data demonstrated the formation of cells with odontoblastic morphologies on existing dentin, suggesting that isolated human pulp stem cells may differentiate into odontoblasts on dentin in vitro.