Investigating distributions and changes of alkaloids in living Catharanthus roseus under low-phosphorus stress using wooden-tip electrospray ionisation mass spectrometry
Investigating distributions and changes of alkaloids in living Catharanthus roseus under low-phosphorus stress using wooden-tip electrospray ionisation mass spectrometry
复制标题
利用木尖电喷雾电离质谱研究低磷胁迫下活长春花中生物碱的分布和变化
DOI：
10.1002/pca.2937
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发表时间：
2020
影响因子：
3.3
通讯作者：
Hu Bin
中科院分区：
文献类型：
--
作者：
Wu Lin;Yao Ya-Nan;Hu Bin
简介长春花生物碱是长春花生产抗癌药物的重要来源。土壤磷素是种植的关键因素之一。目的我们的目标是开发一种与木尖直接质谱联用的体内采样技术，用于研究活体花、叶、茎、脉和根中生物碱的分布和变化。低磷胁迫下的玫瑰。材料和方法LivingC。玫瑰是在低磷胁迫（n = 10）和对照条件（n = 10）下制备的。应用木尖电喷雾电离质谱和常规液相色谱-质谱分析活体C。玫瑰花和提取物 C.结果蛇纹石、长春花碱、长春碱和脱水长春花碱在活体C中的分布和变化。成功获得了低磷胁迫和对照条件下的玫瑰。结论与对照土壤条件相比，低磷土壤诱导了C.玫瑰花在叶、脉、茎和根中产生较多的蛇纹石但较少的长春质和文多灵，并在花、叶、茎和根中产生较多的脱水长春花碱。总的来说，我们的结果展示了一种简单、快速、有效的活体植物体内采样和直接分析方法。
IntroductionVinca alkaloids are important sources for producing anticancer drugs fromCatharanthus roseus. The phosphorus of soil is one of crucial factors for plantingC. roseus.ObjectivesWe aim to develop anin vivosampling technique coupled with direct mass spectrometry with wooden tip for investigating distributions and changes of alkaloids in flowers, leaves, stems, veins and roots of livingC. roseusunder low‐phosphorus stress.Materials and MethodsLivingC. roseuswere prepared under low‐phosphorus stress (n= 10) and control conditions (n= 10). Wooden‐tip electrospray ionisation mass spectrometry and conventional liquid chromatography‐mass spectrometry were applied to analyse livingC. roseusand extracts ofC. roseus, respectively.ResultsDistributions and changes of serpentine, vindoline, catharanthine, and anhydrovinblastine in livingC. roseusunder low‐phosphorus stress and control conditions were successfully obtained.ConclusionCompared to control soil conditions, low‐phosphorus soil was found to induceC. roseusto generate more serpentine but less catharanthine and vindoline in leaves, veins, stems and roots, and to generate more anhydrovinblastine in flowers, leaves, stems and roots. Overall, our results showed a simple, rapid, and effective method forin vivosampling and direct analysis of living plants.