Determination of proteins with α,β,γ,δ-tetrakis(4-sulfophenyl)porphine by measuring the enhanced resonance light scattering at the air/liquid interface
Determination of proteins with α,β,γ,δ-tetrakis(4-sulfophenyl)porphine by measuring the enhanced resonance light scattering at the air/liquid interface
复制标题
DOI：
10.1016/s0003-2670(01)01194-1
复制
发表时间：
2001-09-12
影响因子：
6.2
通讯作者：
Feng, P
中科院分区：
文献类型：
--
作者：
Huang, CZ;Li, YF;Feng, P
通过在普通荧光分光光度计的石英池上安装两个直角棱镜来改变入射/发射光束的传播方向，发展了一种新的检测气液界面光散射信号的技术--反射光散射（RFLS）。在pH 1.86和离子强度0.04，J-和H-聚集的α，β，γ，δ-四（4-磺基苯基）卟啉，发生在蛋白质的存在下，在界面上，通过测量增强共振光散射（RLS）信号，其特征在于在490.2和421.6 nm，分别分配。在490.2 nm处增强的RLS信号下，建立了一种新的蛋白质测定法，其3 σ检测限为18-70 ng ml（-1）。测定了合成样品和人血清中的蛋白质浓度，结果令人满意。(C)2001 Elsevier Science B. V.保留所有权利。
By attaching two right-angled prisms at a quartz cell of a common spectrofluorometer to change the propagation direction of the incident/emission light beams, a novel technique, reflected-light scattering (RFLS), was developed to detect the light scattering signals at the air/liquid interface. At pH 1.86 and ionic strength 0.04, the J- and H-aggregation of alpha,beta,gamma,delta -tetrakis(4-sulfophenyl)porphine that occurred at the interface in the presence of proteins, was assigned by measuring the enhanced resonance light scattering (RLS) signals characterized at 490.2 and 421.6 nm, respectively. With the enhanced RLS signals at 490.2 nm, a novel assay of proteins was established with the 3 sigma limit of detection being 18-70 ng ml(-1). The protein concentrations in synthetic samples and in human serum were determined with satisfactory results. (C) 2001 Elsevier Science B.V. All rights reserved.