Oral commensal bacterial flora is responsible for peripheral differentiation of neutrophils in the oral mucosa in the steady state
Oral commensal bacterial flora is responsible for peripheral differentiation of neutrophils in the oral mucosa in the steady state
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DOI：
10.1016/j.job.2022.11.002
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发表时间：
2023-03-02
影响因子：
2.4
通讯作者：
Itsumi, Momoe
中科院分区：
文献类型：
--
作者：
Trtic, Natasa;Mori, Mina;Itsumi, Momoe
目的：宿主体内的共生细菌在免疫系统的分化和维持中发挥着重要作用。肠道免疫研究表明，在稳态条件下，微生物群落在维持健康方面发挥着重要作用。然而，口腔共生菌对口腔免疫系统的作用仍不清楚。在这里，我们阐明了稳态条件下共生细菌与口腔粘膜免疫系统之间的相互作用。方法：我们使用从未接触过细菌的无菌小鼠和在正常菌群中生长的常规小鼠。从口腔粘膜分离口腔细胞，用特异性抗体染色，并通过流式细胞术进行分析。为了检测髓过氧化物酶和细胞内细胞因子，分别用N-甲酰基-蛋氨酸-亮氨酰-苯丙氨酸和佛波醇12肉豆蔻酸酯13-乙酸酯/离子霉素刺激口腔细胞。 结果：我们发现无菌小鼠的口腔粘膜比常规小鼠的口腔粘膜含有更多的中性粒细胞。然而，无菌口腔粘膜中的大多数中性粒细胞表现出不成熟的表型。无菌小鼠口腔粘膜中的其他免疫细胞，包括巨噬细胞、T 细胞和 B 细胞，显示出与传统小鼠相似的分化。这些结果表明，在稳态口腔粘膜中，正常共生菌群促进中性粒细胞的外周分化。结论：共生菌群的存在对于口腔粘膜中充分免疫系统的发育至关重要。(c) 2022 日本口腔生物学协会。由 Elsevier B.V. 出版。保留所有权利。
Objectives: Commensal bacteria in the host body play a fundamental role in the differentiation and maintenance of the immune system. Studies on intestinal immunity have revealed that, under steadystate conditions, microflora have an important role in the maintenance of health. However, the role of oral commensal bacteria on the oral immune system is still unclear. Here, we clarify the interactions between commensal bacteria and the oral mucosal immune system under steady-state conditions.Methods: We used germ-free mice that had never been exposed to bacteria and conventional mice grown with normal bacterial flora. Oral cells were isolated from the oral mucosa, stained with specific antibodies, and analyzed by flow cytometry. For the detection of myeloperoxidase and intracellular cytokines, oral cells were stimulated with N-formyl-methionine-leucyl-phenylalanine and phorbol 12myristate 13-acetate/ionomycin, respectively.Results: We found that the oral mucosa harbored more neutrophils in germ-free mice than in conventional mice. However, the majority of neutrophils in the germ-free oral mucosa exhibited an immature phenotype. Other immune cells, including macrophages, T cells, and B cells, in the oral mucosa of germ-free mice showed similar differentiation to those in conventional mice. These results indicate that in the steady-state oral mucosa, the normal commensal flora promote the peripheral differentiation of neutrophils.Conclusions: The presence of commensal flora is critical for the development of adequate immune system in the oral mucosa.(c) 2022 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved.