Immunocytochemical localization of a rhodopsin-like protein in the lipochondria in photosensitive neurons of Aplysia californica.
Immunocytochemical localization of a rhodopsin-like protein in the lipochondria in photosensitive neurons of Aplysia californica.
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加州海兔光敏神经元脂软骨中视紫红质样蛋白的免疫细胞化学定位。
DOI：
10.1007/bf00218388
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发表时间：
1986
影响因子：
3.6
通讯作者：
Kegler,LL
中科院分区：
文献类型：
--
作者：
Robles,LJ;Breneman,JW;Anderson,EO;Nottoli,VA;Kegler,LL
针对鱿鱼视蛋白的多克隆抗体被用于免疫细胞化学和免疫印迹实验，以确定视紫红质样蛋白的光敏神经元ofAussia。将醛固定的腹部和大脑神经节包埋在石蜡中进行过氧化物酶抗过氧化物酶分析或整体用于免疫荧光研究。将神经节包埋在Lowicryl K4M中进行电镜免疫细胞化学。在大脑和腹部神经节，光镜免疫细胞化学结果显示，反应产物沉积在周围的神经元细胞周边相应的位置的脂膜。在腹神经节中，巨细胞R2，位于右喙四分之一，和神经元在右尾四分之一一致的抗视蛋白标记。电子显微镜研究表明，铁蛋白标记的lipoprotea在R2和其他免疫反应神经元。R2和脑神经元提取物的免疫印迹分析，用于确定两个突出的免疫反应蛋白带在85000和67500分子量。
Polyclonal antibodies directed against squid opsin were used in immunocytochemical and immunoblot experiments to identify a rhodopsin-like protein in photosensitive neurons ofAplysia. Aldehyde-fixed abdominal and cerebral ganglia were embedded in paraffin for peroxidase anti-peroxidase analysis or used whole for immunofluorescence studies. Ganglia were embedded in Lowicryl K4M for electron-microscope immunocytochemistry. In both the cerebral and abdominal ganglia, light-microscope immunocytochemical results showed reaction product deposited around the neuronal cell periphery corresponding in position to the lipochondria. In the abdominal ganglion, the giant cell R2, located in the right rostral quarter, and neurons in the right caudal quarter were consistently labeled with anti-opsin. Electron-microscopic studies demonstrated ferritin-labeling of the lipochondria in R2 and other immunoreactive neurons. Immunoblot analysis of R2 and cerebral neuron extracts was used to identify two prominent immunoreactive protein bands at 85000 and 67500 molecular weight.