EVIDENCE FOR A NOVEL SPLICE VARIANT OF THE ALPHA-SUBUNIT OF G(O) IN RAT MALE HAPLOID GERM-CELLS
EVIDENCE FOR A NOVEL SPLICE VARIANT OF THE ALPHA-SUBUNIT OF G(O) IN RAT MALE HAPLOID GERM-CELLS
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DOI：
10.1016/0006-291x(92)91606-q
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发表时间：
1992-02-28
影响因子：
3.1
通讯作者：
HANSSON, V
中科院分区：
文献类型：
--
作者：
HAUGEN, TB;ESKILD, W;HANSSON, V
Northern分析表明，大鼠未成熟生殖细胞中Goα mRNA高表达。α o2mrna在粗粒精母细胞中是主要形式，而在单倍体生殖细胞中则大量存在较短链长度的信息。该mRNA与αo2 3′编码区特异的寡核苷酸杂交，但与5′未翻译区和5′编码区特异的寡核苷酸不杂交。结果表明，存在一种新的α omrna剪接变体，它可能编码一种对生殖细胞发育重要的α α蛋白。
Northern analysis shows that Goα mRNA is highly expressed in immature germ cells from rat. Whereasαo2mRNA is the major form in pachytene spermatocytes, a message of shorter chain length is present in large amount in haploid germ cells. This mRNA was detected with an oligonucleotide specific for the 3′-coding region ofαo2, but did not hybridize to oligonucleotides specific for the 5′-untranslated and 5′-coding regions. The results indicate the presence of a novel splice variant ofαomRNA, which may code for a Goα protein important for germ cell development.