Broad geographical distribution and high genetic diversity of shrew-borne Seewis hantavirus in Central Europe
Broad geographical distribution and high genetic diversity of shrew-borne Seewis hantavirus in Central Europe
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DOI：
10.1007/s11262-012-0736-7
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发表时间：
2012-08-01
期刊：
影响因子：
1.6
通讯作者：
Ulrich, Rainer G.
中科院分区：
文献类型：
--
作者：
Schlegel, Mathias;Radosa, Lukas;Ulrich, Rainer G.
很长一段时间以来，汉坦病毒被认为完全是由啮齿动物传播的病原体。最近发现了许多由地鼠和鼹鼠传播的汉坦病毒，这对它们的系统起源提出了重要的问题。本研究的目的是证明在中欧不同的Sorex物种中是否存在和分布与Srew相关的Seewis病毒(SWSV)。因此，在德国、捷克共和国和斯洛伐克共采集到353只蜘蛛、59只微小沙鼠、27只冠状沙鼠和1只高山沙鼠。用汉坦病毒特异性的L片段RT-PCR进行筛选，在353例蜘蛛链球菌和59例微小链球菌中分别检测到49例和4例组织中特异性扩增产物。对45只L片段阳性的蜘蛛和全部4只L片段阳性的微小链球菌进行了S片段的序列测定。对来自德国、捷克共和国和斯洛伐克的这些序列进行的系统发育研究表明，它们与匈牙利、芬兰、奥地利和德国其他地点的SWSV序列相似。低的簇内序列变异性和高的簇间分歧表明在孤立的Sorex种群中存在长期的SWSV进化。在49个SWSV S片段序列中的28个序列中，在编码核衣壳蛋白的相反链上发现了一个额外的开放阅读框架。这是首次对来自德国、捷克和斯洛伐克的SWSV毒株进行全面的序列分析，表明其广泛的地理分布和高度的遗传差异。未来的研究必须证明蜘蛛和微小链球菌是否都代表SWSV的宿主或溢出感染负责在这两个物种中平行的SWSV的分子检测。
For a long time hantaviruses were believed to be exclusively rodent-borne pathogens. Recent findings of numerous shrew- and mole-borne hantaviruses raise important questions on their phylogenetic origin. The objective of our study was to prove the presence and distribution of shrew-associated Seewis virus (SWSV) in different Sorex species in Central Europe. Therefore, a total of 353 Sorex araneus, 59 S. minutus, 27 S. coronatus, and one S. alpinus were collected in Germany, the Czech Republic, and Slovakia. Screening by hantavirus-specific L-segment RT-PCR revealed specific amplification products in tissues of 49 out of 353 S. araneus and four out of 59 S. minutus. S-segment sequences were obtained for 45 of the L-segment positive S. araneus and all four L-segment positive S. minutus. Phylogenetic investigation of these sequences from Germany, the Czech Republic, and Slovakia demonstrated their similarity to SWSV sequences from Hungary, Finland, Austria, and other sites in Germany. The low intra-cluster sequence variability and the high inter-cluster divergence suggest a long-term SWSV evolution in isolated Sorex populations. In 28 of the 49 SWSV S-segment sequences, an additional putative open reading frame (ORF) on the opposite strand to the nucleocapsid protein-encoding ORF was identified. This is the first comprehensive sequence analysis of SWSV strains from Germany, the Czech Republic, and Slovakia, indicating its broad geographical distribution and high genetic divergence. Future studies have to prove whether both S. araneus and S. minutus represent SWSV reservoir hosts or spillover infections are responsible for the parallel molecular detection of SWSV in both species.