L‐α‐Aminooxy‐β‐phenylpropionic acid inhibits lignification but not the differentiation to tracheary elements of isolated mesophyll cells of Zinnia elegans
L‐α‐Aminooxy‐β‐phenylpropionic acid inhibits lignification but not the differentiation to tracheary elements of isolated mesophyll cells of Zinnia elegans
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L-α-氨基氧基-β-苯基丙酸抑制木质化，但不抑制百日草分离叶肉细胞向气管成分的分化
DOI：
10.1111/j.1399-3054.1990.tb08716.x
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发表时间：
1990
影响因子：
6.4
通讯作者：
A. Komamine
中科院分区：
文献类型：
--
作者：
Edgar Ingold;M. Sugiyama;A. Komamine
研究了L-α-氨基氧基-β-苯丙酸（AOPP）作为L-苯丙氨酸解氨酶（PAL; EC 4.3.1.5）抑制剂，对线虫离体叶肉细胞管状分子分化的影响，进而对木质素形成的影响。金丝雀被调查了。低浓度AOPP （5 ~ 25 μ，M）几乎完全阻止了分化细胞的木质化，而分化细胞数、细胞活力、鲜重、干重和堆积细胞体积均高于对照培养的相应参数。高浓度AOPP （50 ~ 75 μM）抑制了气管元件的形成，但仍能观察到细胞的分裂、伸长和扩张。在100 μM AOPP中培养96 h的细胞与加入AOPP培养12 h时的细胞形态相似。在AOPP浓度不抑制分化的情况下，AOPP引起细胞外多糖部分和总细胞壁部分中醛酸和总碳水化合物（每单位体积细胞悬浮液）的数量增加，尽管这些参数在干重基础上表达时与对照值没有显著差异。AOPP可使含木糖的多糖在明显分化开始前释放到培养基中，而含葡萄糖的多糖在二次细胞壁增厚形成时释放到培养基中。结果表明，低浓度的AOPP特异性抑制木质素的形成，而不会对细胞壁多糖的合成产生不利影响，尽管这些化合物与细胞壁的适当整合可能会受到干扰。另一方面，o -苄基羟基胺并没有被证明是一种有效的药物来影响分化百日草细胞的木质素合成。
The influence of L-α-aminooxy-β-phenylpropionic acid (AOPP), an inhibitor of L-phenylalanine ammonia-lyase (PAL; EC 4.3.1.5), and thus of lignin formation, on the differentiation of tracheary elements from isolated mesophyll cells of Zinnia elegans L. cv. Canary Bird was investigated. At low concentrations of AOPP (5–25 μ,M) lignification of differentiating cells was almost completely prevented whereas number of differentiating cells, viability of the cells, fresh and dry weights, and the packed cell volume were higher than corresponding parameters in control cultures. At higher concentrations of AOPP (50–75 μM) the formation of tracheary elements was inhibited but division, elongation and expansion of cells were still observed. Cells cultured for 96 h in the presence of 100 μM AOPP were morphologically similar to cells at 12 h of culture, the time at which AOPP was added. At concentrations of AOPP that did not inhibit differentiation, AOPP caused an increase in the amounts of uronic acid and total carbohydrate (per unit volume of cell suspension) in the extracellular polysaccharide fraction and in the total cell wall fraction, although these parameters were not significantly different from control values when expressed on a dry weight basis. AOPP caused the release of polysaccharides which contained xylose into the medium when added before the onset of visible differentiation and the release of polysaccharides which contained glucose when added at the time when the formation of the secondary cell wall thickenings took place. The results indicate that AOPP at low concentrations specifically inhibits the formation of lignin without adversely affecting the synthesis of cell wall polysaccharides, although the proper integration of these compounds into the wall may be disturbed. O-Benzylhydroxyla-mine, on the other hand, did not prove to be a useful agent to affect lignin synthesis in differentiating Zinnia cells.