Development of Two Types of Rapid Diagnostic Test Kits To Detect the Hemagglutinin or Nucleoprotein of the Swine-Origin Pandemic Influenza A Virus H1N1
Development of Two Types of Rapid Diagnostic Test Kits To Detect the Hemagglutinin or Nucleoprotein of the Swine-Origin Pandemic Influenza A Virus H1N1
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DOI：
10.1128/cvi.00269-10
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发表时间：
2011-03-01
影响因子：
--
通讯作者：
Ikuta, Kazuyoshi
中科院分区：
文献类型：
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作者：
Mizuike, Rika;Sasaki, Tadahiro;Ikuta, Kazuyoshi
自2009年4月出现以来，甲型流感大流行性病毒H1N1 (H1N1 pdm)，一种具有三重重组基因组的新型甲型流感病毒，已在全世界传播。使用免疫层析（IC）诊断患者感染的最初尝试依赖于为季节性甲型和乙型流感病毒开发的检测试剂盒，其中许多病毒被证明对H1N1 pdm的敏感性明显较低。在这里，我们制备了单克隆抗体，与H1N1 pdm反应，但不与季节性流感A （H1N1和H3N2）或B病毒反应。使用其中两种抗体，一种识别病毒血凝素（HA），另一种识别核蛋白（NP），我们开发了用于特异性检测H1N1 pdm的试剂盒，并使用流感样疾病患者的鼻洗液或鼻咽液的临床标本进行了测试。两种IC检测试剂盒的特异性都非常高（HA试剂盒为93%，NP试剂盒为100%）。抗np抗体检测H1N1 pdm的灵敏度为85.5%，抗ha抗体检测灵敏度为49.4%，市售甲型流感病毒检测灵敏度为79.5%。使用抗np抗体可以快速准确地诊断H1N1 pdm感染。
Since its emergence in April 2009, pandemic influenza A virus H1N1 (H1N1 pdm), a new type of influenza A virus with a triple-reassortant genome, has spread throughout the world. Initial attempts to diagnose the infection in patients using immunochromatography (IC) relied on test kits developed for seasonal influenza A and B viruses, many of which proved significantly less sensitive to H1N1 pdm. Here, we prepared monoclonal antibodies that react with H1N1 pdm but not seasonal influenza A (H1N1 and H3N2) or B viruses. Using two of these antibodies, one recognizing viral hemagglutinin (HA) and the other recognizing nucleoprotein (NP), we developed kits for the specific detection of H1N1 pdm and tested them using clinical specimens of nasal wash fluid or nasopharyngeal fluid from patients with influenza-like illnesses. The specificities of both IC test kits were very high (93% for the HA kit, 100% for the NP kit). The test sensitivities for detection of H1N1 pdm were 85.5% with the anti-NP antibody, 49.4% with the anti-HA antibody, and 79.5% with a commercially available influenza A virus detection assay. Use of the anti-NP antibody could allow the rapid and accurate diagnosis of H1N1 pdm infections.