TURBULENT FLUID SHEAR-STRESS INDUCES VASCULAR ENDOTHELIAL-CELL TURNOVER INVITRO
TURBULENT FLUID SHEAR-STRESS INDUCES VASCULAR ENDOTHELIAL-CELL TURNOVER INVITRO
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DOI：
10.1073/pnas.83.7.2114
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发表时间：
1986-04-01
影响因子：
11.1
通讯作者：
GIMBRONE, MA
中科院分区：
文献类型：
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作者：
DAVIES, PF;REMUZZI, A;GIMBRONE, MA
通过将接触抑制的汇合细胞单层暴露于层流或湍流中不同幅度的剪切应力，研究了血液动力学对血管内皮细胞周转率的影响。层流剪切应力（范围，8-15达因/平方厘米; 24小时）诱导细胞排列的流动方向，而不启动细胞周期。相比之下，湍流剪切应力低至1.5达因/厘米2的短时间为3小时刺激大量的内皮细胞DNA合成的细胞排列，可辨别的细胞收缩，或细胞损失的情况下。这些体外实验的结果表明，在动脉粥样硬化病变的血管系统的易发区域，不稳定的血流特性，而不是壁剪切应力本身的大小，可能是血流动力学诱导的内皮细胞更新的主要决定因素。
The effects of hemodynamic forces upon vascular endothelial cell turnover were studied by exposing contact-inhibited confluent cell monolayers to shear stresses of varying amplitude in either laminar or turbulent flow. Laminar shear stresses (range, 8-15 dynes/cm2; 24 hr) induced cell alignment in the direction of flow without initiating the cell cycle. In contrast, turbulent shear stresses as low as 1.5 dynes/cm2 for as short a period as 3 hr stimulated substantial endothelial DNA synthesis in the absence of cell alignment, discernible cell retraction, or cell loss. The results of these in vitro experiments suggest that in atherosclerotic lesion-prone regions of the vascular system, unsteady blood flow characteristics, rather than the magnitude of wall shear stress per se, may be the major determinant of hemodynamically induced endothelial cell turnover.