Transplantation of tissue-cultured corneal endothelium.
Transplantation of tissue-cultured corneal endothelium.
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组织培养的角膜内皮移植。
DOI：
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发表时间：
1978
影响因子：
4.4
通讯作者：
J. McCulley
中科院分区：
文献类型：
--
作者：
M. Jumblatt;D. Maurice;J. McCulley
培养的内皮细胞在兔眼中被替换后，已经被证明恢复了它们的生理功能。去除角膜中的天然内皮细胞，然后种植培养细胞。经过一段潜伏期后，从这些角膜上切下全厚度的纽扣，并移植到受体动物体内。只有当供体细胞来自不到一个月大的培养物时，才能获得透明的移植物。光镜和扫描电子显微镜显示，这些移植物的内皮细胞在角膜后表面呈轻微不规则的单层。在苯扎氯铵引起的角膜水肿中，透明的移植物仍然被厚厚的、浑浊的宿主组织所包围。在有~3H-胸腺嘧啶核苷标记细胞的移植物中，放射性仅限于宿主组织。
Cultured endothelial cells have been shown to regain their physiological function when replaced in the rabbit eye. Corneas were wiped free of native endothelium and seeded with cultured cells. After an incubation period, full-thickness buttons were cut from these corneas and transplanted into recipient animals. Clear grafts were obtained only when the donor cells were derived from cultures less than a month old. Light and scanning electron microscopy showed the endothelial cells of these grafts to be present as a slightly irregular monolayer on the posterior surface of the cornea. In corneas made edematous by benzalkonium chloride, the clear graft remained surrounded by thick and cloudy host tissue. In those grafts with 3H-thymidine--labeled cells, radioactivity was limited to the host tissue.