Cyclin E and cyclin A as candidates for the restriction point protein.
Cyclin E and cyclin A as candidates for the restriction point protein.
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DOI：
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发表时间：
1993-04
期刊：
影响因子：
11.2
通讯作者：
Q. Dou;A. Levin;Shanchuan Zhao;A. Pardee
中科院分区：
文献类型：
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作者：
Q. Dou;A. Levin;Shanchuan Zhao;A. Pardee
细胞进入S期的进程被认为是由不稳定蛋白质（限制点蛋白R; A. B。Pardee，Proc. Natl. Acad. Sci. USA，71：1286-1290，1974）。我们在这里报告，细胞周期蛋白E和细胞周期蛋白A蛋白，以及它们的依赖组蛋白H1激酶满足所有的标准的R蛋白，其中包括后期G1期增加，过度延迟后的外观抑制蛋白质合成在非转化细胞，和更快的恢复转化细胞。我们认为R蛋白的分子基础可能是细胞周期蛋白的产生和失活。
Progression of cells into S phase is proposed to be determined by accumulation of a labile protein (the restriction point protein R; A. B. Pardee, Proc. Natl. Acad. Sci. USA, 71: 1286-1290, 1974). We report here that cyclin E and cyclin A proteins as well as their dependent histone H1 kinases satisfy all of the criteria for the R protein, which includes late G1 phase increase, an excess delay of appearance after inhibition of protein synthesis in nontransformed cells, and a faster recovery in transformed cells. We suggest that the molecular basis of the R protein could be cyclin production and inactivation.