Optimization of subculture and DNA extraction steps within the whole genome sequencing workflow for source tracking of Salmonella enterica and Listeria monocytogenes
Optimization of subculture and DNA extraction steps within the whole genome sequencing workflow for source tracking of Salmonella enterica and Listeria monocytogenes
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DOI：
10.1016/j.mimet.2018.06.005
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发表时间：
2018-08-01
影响因子：
2.2
通讯作者：
Baert, Leen
中科院分区：
文献类型：
--
作者：
Gimonet, Johan;Portmann, Anne-Catherine;Baert, Leen
这项工作表明，将培养时间减少到4-5小时，准备DNA提取，然后进行自动DNA提取，可以缩短动手时间，周转时间30%，提高吞吐量，同时保持高质量单核苷酸多态性分析评估的WGS质量。
This work shows that an incubation time reduced to 4-5 h to prepare a culture for DNA extraction followed by an automated DNA extraction can shorten the hands-on time, the turnaround time by 30% and increase the throughput while maintaining the WGS quality assessed by high quality Single Nucleotide Polymorphism analysis.