Effects of nanoparticle-mediated growth factor gene transfer to the injured microenvironment on the tendon-to-bone healing strength
Effects of nanoparticle-mediated growth factor gene transfer to the injured microenvironment on the tendon-to-bone healing strength
复制标题
纳米颗粒介导的生长因子基因转移到受伤微环境对肌腱-骨愈合强度的影响
DOI：
10.1039/d0bm01222j
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发表时间：
2020-12-07
影响因子：
6.6
通讯作者：
Tang，Jin Bo
中科院分区：
文献类型：
--
作者：
Xing，Shu Guo;Zhou，You Lang;Tang，Jin Bo
创伤后的肌腱-骨愈合通常缓慢而脆弱，并且在早期的活动锻炼中容易破坏修复部位。bFGF和VEGFA基因治疗可能通过增强细胞增殖和血管生成来增强肌腱-骨愈合过程。进行本研究以确定纳米颗粒介导的bFGF和VEGFA基因共递送至肌腱-骨修复界面对鸡模型中的愈合强度和生物反应的影响。制备了PLGA纳米粒/pEGFP-bFGF + pEGFP-VEGFA质粒复合物，并进行了体内外表征。纳米颗粒/质粒复合物可以有效地将bFGF和VEGFA基因转移到肌腱-骨界面。纳米颗粒介导的bFGF和VEGFA基因的共递送在鸡屈肌腱修复模型中的愈合强度和组织学方面显著改善了肌腱-骨愈合。我们的研究结果提出了一种新的生物学方法来加速肌腱-骨愈合。
The tendon-to-bone healing after trauma is usually slow and weak, and the repair site is easily disrupted during early mobilization exercise. bFGF and VEGFA gene therapy may hold promise in augmenting the tendon-to-bone healing process through enhancing cell proliferation and angiogenesis. This study is conducted to determine the effects of nanoparticle-mediated co-delivery of bFGF and VEGFA genes to the tendon-to-bone repair interface on the healing strength and biological responses in a chicken model. The PLGA nanoparticle/pEGFP-bFGF + pEGFP-VEGFA plasmid complexes were prepared and were characterized in vitro and in vivo. The nanoparticle/plasmid complexes can effectively transfer bFGF and VEGFA genes to the tendon-to-bone interface. Nanoparticle-mediated co-delivery of bFGF and VEGFA genes significantly improved the tendon-to-bone healing in terms of healing strengths and histology in a chicken flexor tendon repair model. Our results suggest a new biological approach to accelerate the tendon-to-bone healing.