Local in vitro delivery of rapamycin from electrospun PEO/PDLLA nanofibers for glioblastoma treatment
Local in vitro delivery of rapamycin from electrospun PEO/PDLLA nanofibers for glioblastoma treatment
复制标题
电纺 PEO/PDLLA 纳米纤维体外局部递送雷帕霉素用于胶质母细胞瘤治疗
DOI：
10.1016/j.biopha.2016.08.033
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发表时间：
2016
影响因子：
7.5
通讯作者：
Ni Shilei
中科院分区：
文献类型：
--
作者：
Wang Benlin;Li Haoyuan;Yao Qingyu;Zhang Yulin;Zhu Xiaodong;Xia Tongliang;Wang Jian;Li Gang;Li Xingang;Ni Shilei
雷帕霉素是雷帕霉素抑制剂和抗增殖剂的哺乳动物靶标，用于治疗神经胶质瘤和其他恶性肿瘤，但其有效性受到其不能以靶向方式递送至肿瘤部位的事实的限制。为了解决这个问题，我们通过静电纺丝使用两种可生物降解的材料，聚乳酸（PLA）和聚环氧乙烷（PEO）作为载体雷帕霉素输送到肿瘤制造了一个网。纳米纤维直径随着混合溶液中PLA浓度的增加而减小。扫描电子显微镜分析表明，混杂纤维的表面形态光滑均匀。傅立叶变换红外光谱分析表明，雷帕霉素被封装在聚合物溶液中，封装效率高，在药物浓度范围内稳定从0.5-2重量%。观察到体外药物缓释与培养胶质瘤细胞的细胞毒性之间的相关性。这些结果表明，PEO/聚（d，l-乳酸）网状物可用作雷帕霉素的靶向递送系统，其可限制副作用并防止神经胶质瘤手术切除后的局部复发。
Rapamycin, a mammalian target of rapamycin inhibitor and anti-proliferative agent, is used to treat glioma and other malignancies, but its effectiveness is limited by the fact that it cannot be delivered in a targeted manner to the site of the tumor. To address this issue, we fabricated a mesh via electrospinning using two biodegradable materials, poly(lactic acid) (PLA) and polyethylene oxide (PEO) as a carrier for rapamycin delivery to the tumor. Nanofiber diameter decreased with increasing PLA concentration in the mixed solution. Scanning electron microscopy analysis revealed the smooth and uniform surface morphology of hybrid fibers. Fourier transform infrared spectroscopy analysis demonstrated that rapamycin was encapsulated in the polymer solution; encapsulation efficiency was high and stable over the range of drug concentrations from 0.5–2 wt%. A correlation was observed between sustained release of the drug in vitro and cytotoxicity in cultured glioma cells. These results indicate that the PEO/poly(d,l-lactic acid) nanofiber mesh can be used as a targeted delivery system for rapamycin that can limit side effects and prevent locoregional recurrence following surgical resection of glioma.